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Automated colorimetric assay of Norgestrel
Steroids, 1970Abstract A relatively sensitive assay for the contraceptive steroid norgestrel, in pharmaceutical preparations, is described. The procedure involves the blue tetrazolium reaction. Optimum conditions for color development were established. The relative standard deviation for the assay, which has a sample rate of 30 hr−1, were of the order of 0.9% at a
P M, Short, C T, Rhodes
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Colorimetric assay of catalase
Analytical Biochemistry, 1972A simple colorimetric assay for catalase activity has been described using K2Cr2O7/acetic acid reagent. Kat. f values of different enzyme sources were determined by the colorimetric method and compared with the values obtained by titrimetric methods.
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An automated colorimetric mutarotase assay
Analytical Biochemistry, 1966Abstract A fully automated mutarotase assay capable of measuring thirty samples per hour or monitoring the effluent from a column is presented. A procedure for partially purifying tissue extracts for the assay is described. Comparisons with a polarimetric assay are given.
J B, Hill, D S, Cowart
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Colorimetric Assay of Tetracycline Antibiotics
Journal of Pharmaceutical Sciences, 1971A sensitive colorimetric method of analysis for seven compounds of the tetracycline series was developed, based on the formation of a colored complex between the tetracyclines and quadrivalent thorium. The effects of pH and time upon the stability of the complexes were observed.
L G, Chatten, S I, Krause
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Detection of opines by colorimetric assay
Analytical Biochemistry, 1987A colorimetric procedure for confirming the presence of arginine-derived opines (nopaline and octopine) in plant tissue extracts is described. Those materials are widely used as markers of plant cell transformation and tumorigenesis mediated by the tumor-inducing plasmids of Agrobacterium tumefaciens.
N S, Yang, S G, Platt, P, Christou
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An improved colorimetric assay for polyols
Analytical Biochemistry, 1977Abstract A new assay for polyols was developed that minimizes interference by sugars. It is based on oxidation of alditols to formaldehyde by acidic periodate, reduction of the excess periodate with l -rhamnose, and a short-time determination of the formaldehyde with the Nash reagent. When glycerol was added to various crude biological materials and
S H, Bok, A L, Demain
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An easy colorimetric assay for glycosyltransferases
Biochemistry (Moscow), 2010Glycosyltransferases are involved in biosynthesis of both protein-bound and non-bound glycans that have multiple and important biological functions in all species. A variety of methods for assaying glycosyltransferase activity have been developed driven by the specific interests and type of information required by researchers.
Rui, Shen +6 more
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A quantitative colorimetric assay for semialdehydes
Analytical Biochemistry, 1990We have studied the ability of the enzyme pyrroline-5-carboxylate dehydrogenase to oxidize aldehydes which are structurally similar to pyrroline-5-carboxylate, the enamine of gamma-glutamyl semialdehyde. These studies required the organic synthesis of semialdehydes not commercially available.
W C, Small, M E, Jones
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A colorimetric formaldehyde assay
Applied Biochemistry and Biotechnology, 1997A room-temperature assay of formaldehyde is described. The assay uses few reagents and is colorimetric, read at a wavelength of 649 nm. Tryptophan and tryptamine were noted as interfering with the assay, probably by binding with the formaldehyde. High levels of sugar show smaller effects on final absorbance. Glyceraldehyde also reacts in the assay, but
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Colorimetric Assay for Acetaminophen in Serum
Therapeutic Drug Monitoring, 1984A colorimetric method for determination of acetaminophen in serum, based on its reaction with 2-nitroso-1-naphthol-4-sulfonic acid, is described. The method is sensitive, rapid, and free from the interferences of serum matrix and most common drugs. The method can be performed manually or can be semiautomated. The results from this method correlate well
Z K, Shihabi, R M, David
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