Results 81 to 90 of about 190,909 (304)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

The determination of N-methylcarbamate pesticides using enzyme immunoassays with chemiluminescent detection

Czech Journal of Food Sciences, 2004
In the present work, enzyme-linked immunosorbent assays (ELISAs) with chemiluminescent detection for the determination of carbofuran, carbaryl and methiocarb were developed and the analytical parameters of these assays were compared with those of ELISAs ...
B. Mičková, P. Rauch, A. Montoya, E. Ferri, F. Fini, S. Girotti   +5 more
doaj   +1 more source

ALS With and Without Upper Motor Neuron Signs: A Comparative Study Supporting the Gold Coast Criteria

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective The Gold Coast criteria permit diagnosis of amyotrophic lateral sclerosis (ALS) even without upper motor neuron (UMN) signs. However, whether ALS patients with UMN signs (ALSwUMN) and those without (ALSwoUMN) share similar characteristics and prognoses remains unclear.
Hee‐Jae Jung, E‐nae Cheong, Jungmin So, Heung‐Won Kang, Yangsean Choi, Eun‐Jae Lee, Hyunjin Kim, Young‐Min Lim   +7 more
wiley   +1 more source

Accuracy of Blood Loss Measurement during Cesarean Delivery

American Journal of Perinatology Reports, 2017
Objective This study aims to compare the accuracy of visual, quantitative gravimetric, and colorimetric methods used to determine blood loss during cesarean delivery procedures employing a hemoglobin extraction assay as the reference standard.
Sahar V. Doctorvaladan, Andrea T. Jelks, Eric W. Hsieh, Robert L. Thurer, Mark I. Zakowski, David C. Lagrew   +5 more
doaj   +1 more source

ALDOA Promotes Glycolysis and NLRP3/GSDMD Pyroptosis to Accelerate ALS Progression

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration. Glycolytic dysregulation is implicated in disease progression, yet the underlying mechanisms remain unclear. This study investigates how Aldolase A (ALDOA) drives ALS progression through glycolysis‐mediated motor neuron pyroptosis.
Kaixin Yan, Yan Jiang, Yuxuan Yong, Tianshuo Zhang, Niannian Zhang, Qianqian Zeng, Xue Gong, Li Meng, Fangfang Bi, Yongmin Liu   +9 more
wiley   +1 more source

Stable, covalent attachment of laminin to microposts improves the contractility of mouse neonatal cardiomyocytes. [PDF]

, 2014
The mechanical output of contracting cardiomyocytes, the muscle cells of the heart, relates to healthy and disease states of the heart. Culturing cardiomyocytes on arrays of elastomeric microposts can enable inexpensive and high-throughput studies of ...
Ashley, Euan A, Pruitt, Beth L, Ribeiro, Alexandre JS, Zaleta-Rivera, Kathia   +3 more
core   +2 more sources

Light Activated Induction of Cuproptosis in Resistant Cancer Cells Using Polymeric BODIPY Nanoparticles for Photoactivated Chemotherapy

Advanced Functional Materials, EarlyView.
This article presents a photo‐responsive nanoparticle platform that precisely triggers copper‐dependent cuproptotic cell death in (drug‐resistant) cancer cells. The system remains stable and inactive in the dark but releases cytotoxic species upon red‐light irradiation, achieving potent activity in drug‐resistant breast cancer cells.
Ricarda Zimmermann, Nicolás Montesdeoca, Johannes Karges   +2 more
wiley   +1 more source

Colorimetric detection of the potent carcinogen aflatoxin B1 based on the aggregation of L-lysine-functionalized gold nanoparticles in the presence of copper ions

Frontiers in Nutrition
L-lysine functionalized gold nanoparticles (AuNPs-Lys) have been widely used for the detection of worldwide interest analytes. In this work, a colorimetric assay for the detection of the carcinogen aflatoxin B1 (AFB1) based on the aggregation of AuNPs ...
Kaori Sánchez-Carrillo, David Quintanar-Guerrero, Miguel José-Yacamán, Abraham Méndez-Albores, Alma Vázquez-Durán, Alma Vázquez-Durán   +5 more
doaj   +1 more source

Direct replacement of antibodies with molecularly imprinted polymer (MIP) nanoparticles in ELISA - development of a novel assay for vancomycin [PDF]

, 2013
A simple and straightforward technique for coating microplate wells with molecularly imprinted polymer nanoparticles (nanoMIPs) to develop ELISA type assays is presented here for the first time.
Caygill, J. S., Chianella, Iva, Guerreiro, Antonio, Moczko, Ewa, Perez De Vargas Sansalvador, Isabel M., Piletska, Elena V., Piletsky, Sergey A., Whitcombe, Michael J.   +7 more
core   +1 more source