Results 151 to 160 of about 262,925 (314)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Removal of colour from solutions of azo dyes using bacterial cells (Shewanella strain J18 143) [PDF]

, 2007
Water pollution control is presently one of the major areas of scientific activity. While coloured organic compounds generally impart only a minor fraction of the organic load to wastewater, their colour renders them aesthetically unacceptable.
Li, Tie
core  

Preliminary Quantitative Evaluation of the Optimal Colour System for the Assessment of Peripheral Circulation from Applied Pressure Using Machine Learning

Sensors
Peripheral circulatory failure refers to a condition in which the blood flow through superficial capillaries is markedly reduced or completely occluded. In clinical practice, nurses strictly adhere to regular repositioning protocols to prevent peripheral
Masanobu Tsurumoto, Takunori Shimazaki, Jaakko Hyry, Yoshifumi Kawakubo, Takeshi Yokoyama, Daisuke Anzai   +5 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Enhancing the soft proofing paradigm [PDF]

, 2007
The first part of this study is devoted to assessing the suitability of soft proofs as a surrogate for the final print in judging colour-reproduction quality. Two sets of psychophysical experiments were carried out.
Gatt, Alexis
core  

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

The Aesthetic of Opulence: Channelling Colour and Darkness in Early Costume and Fashion Film [PDF]

, 2019
Colour was unquestionably one of the key elements in the early displays of dress in cinema, and vice versa: costume was from the beginning a central cinematic device through which to showcase colour.
Uhlirova, Marketa
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Effect of extrinsic and intrinsic factors on colour stability of 3D-printed, thermoplastic, and conventional resin materials: an in vitro study

Saudi Dental Journal
The colour stability of occlusal devices is a critical factor in their long-term aesthetic performance. This in vitro study aimed to evaluate and compare the colour stability of 3D-printed, thermoplastic, and conventional resin materials used in occlusal
Azita Mazaheri Tehrani, Arash Zarbakhsh, Alireza Shafigh, Somayeh Hashemi   +3 more
doaj   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source