Results 121 to 130 of about 15,848 (274)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

A Continuous Centennial Late Glacial-Early Holocene (15-10 cal kyr BP) Palynological Record from the Iberian Pyrenees and Regional Comparisons. [PDF]

Plants (Basel), 2023
Rull V, Blasco A, Calero MÁ, Blaauw M, Vegas-Vilarrúbia T.   +4 more
europepmc   +1 more source

Nota suplementària a "Problemes de cronologia lul·liana" [PDF]

, 1977
not ...
Bonner, Anthony
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

A fast-growing basal troodontid (Dinosauria: Theropoda) from the latest Cretaceous of Europe. [PDF]

Sci Rep, 2021
Sellés AG, Vila B, Brusatte SL, Currie PJ, Galobart À.   +4 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Local reports of climate change impacts in Sierra Nevada, Spain: sociodemographic and geographical patterns. [PDF]

Reg Environ Change, 2023
García-Del-Amo D, Mortyn PG, Reyes-García V.   +2 more
europepmc   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Recognizing Indigenous peoples' and local communities' rights and agency in the post-2020 Biodiversity Agenda. [PDF]

Ambio, 2022
Reyes-García V, Fernández-Llamazares Á, Aumeeruddy-Thomas Y, Benyei P, Bussmann RW, Diamond SK, García-Del-Amo D, Guadilla-Sáez S, Hanazaki N, Kosoy N, Lavides M, Luz AC, McElwee P, Meretsky VJ, Newberry T, Molnár Z, Ruiz-Mallén I, Salpeteur M, Wyndham FS, Zorondo-Rodriguez F, Brondizio ES.   +20 more
europepmc   +1 more source