Results 71 to 80 of about 2,889 (232)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Irredundant bases for finite almost simple groups [PDF]

In this thesis, we study maximum irredundant base sizes I(G, Γ) of three types of finite primitive permutation groups G, where two of the three types are almost simple.
Wu, Peiran
core   +1 more source

Análisis de rutas biológicas y estudio de asociación amplia del genoma identificaron factores genéticos del cromosoma 10 asociados con la variación de antocianinas en papa [PDF]

, 2019
ilustraciones, gráficas, tablasPotato is considered a basic crop worldwide and is the third source of antioxidants in the human diet, due to its per capita consumption.
Parra Galindo, Maria Angelica
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Uncountable groups in which all large subgroups are permutably complemented

Rendiconti del Seminario Matematico della Università di Padova
A subgroup H of a group G is permutably complemented if it admits a permutable complement, that is, a subgroup K
openaire   +1 more source

Polymorphisms and Haplotypes in the XRCC1 Gene and the Risk of Advanced Non-Small Cell Lung Cancer

, 2010
Introduction and HypothesisSingle-nucleotide polymorphisms (SNPs) and haplotypes in the x-ray repair cross-complementing group 1 (XRCC1) gene, which is one of the base excision repair genes, are thought to be associated with the risk of non-small cell ...
Kwon, Hyuk-Chan, Sung-Hyun Kim, Kim, Sunjoo, Kim, Sung-Hyun, Gyeong-Won Lee, Sung Yong Oh, Oh, Sung Yong, Hyuk-Chan Kwon, Kim, Dong Chul, Sunjoo Kim, Kim, Hoon-Gu, Dong Chul Kim, Lee, Gyeong-Won, Kim, In-Suk, Hoon-Gu Kim, In-Suk Kim   +15 more
core   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Integrative radiomics expression predicts molecular subtypes of primary clear cell renal cell carcinoma

, 2018
AIM: To identify combined positron-emission tomography (PET)/magnetic resonance imaging (MRI)-based radiomics as a surrogate biomarker of intratumour disease risk for molecular subtype ccA and ccB in patients with primary clear cell renal cell carcinoma (
Khandani, A.H., Brooks, S.A., Wallen, E.M., Wang, L., Fielding, J.R., Shen, L., Yin, Q., Rathmell, W.K., Lin, W., Milowsky, M.I., Shen, D., Hung, S.-C., Woods, M.E.   +12 more
core   +1 more source