Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley +1 more source
Optimizing Medicare Annual Wellness Visits Through Quality Improvement: Leveraging Process, Continuity, and Combined Visits. [PDF]
Wellman CD +5 more
europepmc +1 more source
Preparation and characterization of renal cell peptides from fetal rats for their antitumor activity
This study aimed to prepare renal cells (RCs) from fetal rats which were digested by enzymes. Candidate peptides RCPs were characterized by capillary HPLC and MS and their bioactivity was predicted using peptideranker. The predicted top 10 bioactive peptides were synthesized.
Zhe Zhang +6 more
wiley +1 more source
How long does it take to complete and publish a systematic review of animal studies? [PDF]
Bugajska JV +6 more
europepmc +1 more source
Unique Completion Techniques Utilized In A Multi-Zone Waterflood Project
W.C. Ireland, L.B. Deckert
openalex +1 more source
Phenotypic plasticity in a newly established set of EGFR inhibitor‐adapted NSCLC cell lines during adaptation and in established cell lines. Here, we introduce novel sublines of the EGFR‐mutant non‐small cell lung cancer (NSCLC) cell lines HCC827 and HCC4006 adapted to the EGFR kinase inhibitors gefitinib (HCC827rGEFI2μm, HCC4006rGEFI1μm), erlotinib ...
Tharsagini V. Nanthaprakash +6 more
wiley +1 more source
A Novel Method of Coupling In Situ Time-Resolved FTIR and Microwave Irradiation: Application to the Monitoring of Quinoxaline Derivatives Synthesis. [PDF]
Cherniienko A +5 more
europepmc +1 more source
The Completion and Transcriptions of Bishamondo School's Textbook
Kanchu Ogami
openalex +2 more sources
The E3 ubiquitin ligase, RNF219, suppresses CNOT6L expression to exhibit antiproliferative activity
We identified RNF219 as a CCR4‐NOT complex‐interacting E3 ubiquitin ligase that targets the CCR4‐NOT subunit CNOT6L for ubiquitination. RNF219 directly binds to the DUF3819 domain of CNOT1 through its putative α‐helix spanning amino acids 521–542. Our findings also suggest that antiproliferative activity of RNF219 is at least partially mediated by ...
Shou Soeda +9 more
wiley +1 more source
Nutritional Status and Chemotherapy Completion in Resectable Pancreatic Cancer: A Narrative Review. [PDF]
Funamizu N +6 more
europepmc +1 more source

