RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Biomechanical behavior of fiber-reinforced and bulk-fill composites in extensive class II restorations: a three-dimensional finite element analysis. [PDF]
Alarco-Jurado LC +2 more
europepmc +1 more source
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Preparation of HMX/PMMA Composite Microspheres with Excellent Properties by Photoinitiated Emulsion Polymerization. [PDF]
Zhang S +7 more
europepmc +1 more source
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Lightweight Structural Design of UAM Fuselage Using AI Predictive Modeling and Composite Big Data from Automated Manufacturing. [PDF]
Son WH, Kim JH, Bae SY.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Composite Ultrasound Score Integrating VExUS Grading and Right Ventricular Function for Predicting 90-Day Heart Failure Rehospitalization: A Single-Center Retrospective Cohort Study. [PDF]
Tang H +6 more
europepmc +1 more source
A Dusty Affair: SIRT1-S682 Modulation Orchestrates ERK-FN1-p38-NF-κB Signaling and Composite-Dependent IL-8 Responses in Gingival Keratinocytes Exposed to Dental Dust and Eluates. [PDF]
Bai S +4 more
europepmc +1 more source
Lifestyle factors moderate emotion-gait coupling in young adults. [PDF]
Wadsworth DD +5 more
europepmc +1 more source

