Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho +3 more
wiley +1 more source
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
THE RELATION BETWEEN THE INTENSITY OF THE USE OF COMPUTER LABORATORY FACILITY AND LEARNING MOTIVATION AND LEARNING RESULT OF INFORMATION AND COMMUNICATION TECHNOLOGY (TIK) OF GRADE XI COMPUTER AND NETWORK ENGINEERING IN SMK NEGERI 2 DEPOK SLEMAN ACADEMIC YEAR 2012/2013 [PDF]
This study aimed to discover (1) the relation between intensity of the use of computer laboratory facility and TIK learning result of grade 11 computer and network engineering students of SMKN 2 Depok Sleman Academic Year 2012/2013, (2) the relation ...
Debbie Ahmad, Nusyera
core
An intracellular transporter mitigates the CO2‐induced decline in iron content in Arabidopsis shoots
This study identifies a gene encoding a transmembrane protein, MIC, which contributes to the reduction of shoot Fe content observed in plants under elevated CO2. MIC is a putative Fe transporter localized to the Golgi and endosomal compartments. Its post‐translational regulation in roots may represent a potential target for improving plant nutrition ...
Timothy Mozzanino +7 more
wiley +1 more source
Mobile learning has started to perform an increasingly significant role in improving learning outcomes in education. Successful and efficient implementation of m-learning in higher education, as with all educational levels, depends on users’ acceptance ...
D. Gezgin +2 more
semanticscholar +1 more source
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Introducing ‘Neuromorphic Computing and Engineering’
Abstract The standard nature of computing is currently being challenged by a range of problems that start to hinder technological progress. One of the strategies being proposed to address some of these problems is to develop novel brain-inspired processing methods and technologies, and apply them to a wide range of application ...
openaire +2 more sources
By dawn or dusk—how circadian timing rewrites bacterial infection outcomes
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo +2 more
wiley +1 more source
Using Computer Technology Tools to Improve the Teaching-Learning Process in Technical and Vocational Education: Mechanical Engineering Subject Area [PDF]
This paper discusses the integration of computer assisted instructions (CAI) with traditional class room teaching. It describes a teaching method to bring real-world of industrial work into the classroom that underscores the need to learn fundamental ...
Abdulrasool, Salah Mahdi, Mishra, Rakesh
core +1 more source
Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes +3 more
wiley +1 more source

