Results 201 to 210 of about 202,879 (264)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Assessing internal construct validity of DAPSA and DAPSA28 in psoriatic arthritis: a European observational study using confirmatory factor analysis and additional psychometric testing. [PDF]

RMD Open
Jørgensen JB, Christensen KB, Michelsen B, Loft AG, Horskjær Rasmussen S, Heberg J, Hetland ML, Zavada J, Pavelka K, Iannone F, Conti F, Borges J, Codreanu C, Mogosan C, Glintborg B, Ciurea A, Nissen MJ, Nordström D, Kuusalo L, Laas K, Vorobjov S, Gudbjornsson B, Love TJ, Østergaard M, Ørnbjerg LM.   +24 more
europepmc   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Construct Validity of the Staff Resource Adequacy Questionnaire for Healthcare Professionals (SRAQ-HP): An Exploratory and Confirmatory Factor Analysis from Latvia. [PDF]

Nurs Rep
Cerela-Boltunova O, Millere I, Trups-Kalne I.   +2 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Development and validation of the socially shared regulated learning questionnaire: insights from a second-order confirmatory factor analysis. [PDF]

Front Psychol
Yang Y, He Z, Wei Y, Tang E.
europepmc   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Examining psychometric properties of the Sinhala version of the 'Opening Minds Scale for Health Care Providers' to measure stigma towards mental illness using confirmatory factor analysis. [PDF]

BMC Res Notes
Samaranayake A, Baminiwatta A, Ranasinghe K, Suraweera C.   +3 more
europepmc   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Development of a brief form of the Chinese version of the Odor Awareness Scale(OAS-B): A confirmatory factor analysis approach to item reduction. [PDF]

PLoS One
Zhang B, Li X, He M, Huang S, Huang S, Wang L, Cao L, Xu H, Nie G.   +8 more
europepmc   +1 more source