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Current Protocols in Cell Biology, 1997
AbstractThis comprehensive overview unit introduces the reader to confocal microscopy from the basic principles of imaging and optical sectioning, to selection of laser, practical guidelines for fixation, choice of fluorophore, control samples, and mounting the sample. There are also suggestions for optimizing the imaging parameters.
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AbstractThis comprehensive overview unit introduces the reader to confocal microscopy from the basic principles of imaging and optical sectioning, to selection of laser, practical guidelines for fixation, choice of fluorophore, control samples, and mounting the sample. There are also suggestions for optimizing the imaging parameters.
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Modern Laser Scanning Confocal Microscopy
Current Protocols in Cytometry, 2018Since its commercialization in the late 1980's, confocal laser scanning microscopy (CLSM) has since become one of the most prevalent fluorescence microscopy techniques for three‐dimensional structural studies of biological cells and tissues.
P. Bayguinov +5 more
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Confocal Microscopy in Ophthalmology
American Journal of Ophthalmology, 2009To describe the principles, capabilities, and applications of confocal microscopy in vivo in ophthalmology.Perspective, literature review, and commentary.Review and synthesis of selected recent literature, with interpretation and perspective.Confocal microscopy imaging has led to a better understanding of the cellular microstructure in the normal ...
Sanjay V. Patel +2 more
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Cell Biology and Toxicology, 1994
Confocal microscopy is an excellent method for studying the localization of fluorescent stains. Used in this way, superior 3D images can be obtained from multiple optical sections with very shallow depth of field. The main advantage of this technique is that the sample is not damaged.
D. Black +5 more
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Confocal microscopy is an excellent method for studying the localization of fluorescent stains. Used in this way, superior 3D images can be obtained from multiple optical sections with very shallow depth of field. The main advantage of this technique is that the sample is not damaged.
D. Black +5 more
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2011
Dermatologic diagnosis is primarily based on interpretation of morphological information by visual inspection, confirmed by histopathological diagnosis if necessary. The challenge is to establish a correct diagnosis and to identify all malignant lesions while minimizing unnecessary surgical procedures.
Wurm, Elisabeth M. T., Soyer, Hans Peter
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Dermatologic diagnosis is primarily based on interpretation of morphological information by visual inspection, confirmed by histopathological diagnosis if necessary. The challenge is to establish a correct diagnosis and to identify all malignant lesions while minimizing unnecessary surgical procedures.
Wurm, Elisabeth M. T., Soyer, Hans Peter
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Scanning confocal optical microscopy and magnetic resonance on single defect centers
, 1997The fluorescence of individual nitrogen-vacancy defect centers in diamond was observed with room-temperature scanning confocal optical microscopy. The centers were photostable, showing no detectable change in their fluorescence emission spectrum as a ...
A. Gruber +5 more
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Confocal microscopy of the cornea
Progress in Retinal and Eye Research, 1999This paper provides the clinician and the researcher with an in-depth manual on the use of a scanning-slit confocal light microscope for the clinical examination and investigation of the living human cornea in vivo. The scope of the paper includes a thorough explanation of the principles of various types of confocal microscopes as well as their ...
Barry R. Masters, Matthias Böhnke
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Reflectance Confocal Microscopy
2015The ultimate goal of early melanoma diagnosis is the detection of all melanomas, while minimizing the number of excisions of nevi. To achieve this goal requires the clinician to not only be able to identify specific melanoma features but to also recognize the distinct aspects of nevi. To complicate the matter, several pigmented and nonpigmented lesions
LONGO, Caterina, PELLACANI, Giovanni
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Confocal Microscopy on the Internet
2013In a few short years, the Internet (in terms of the World Wide Web) has become a powerful informational resource for the original scientific literature pertaining to biological investigations using the laser scanning confocal microscope. However, there still remains an obvious void in the development of educational Web sites targeted at beginning ...
Ericka B. Ramko +8 more
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Hyperspectral confocal microscope
Applied Optics, 2006We have developed a new, high performance, hyperspectral microscope for biological and other applications. For each voxel within a three-dimensional specimen, the microscope simultaneously records the emission spectrum from 500 nm to 800 nm, with better than 3 nm spectral resolution.
Howland D. T. Jones +3 more
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