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A video rate laser scanning confocal microscope
SPIE Proceedings, 2008A video-rate laser scanning microscope was developed as an imaging engine to integrate with other photonic building blocks to fulfill various microscopic imaging applications. The system is quipped with diode laser source, resonant scanner, galvo scanner, control electronic and computer loaded with data acquisition boards and imaging software. Based
Hongzhou Ma +3 more
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Spherical-coordinate scanning confocal laser microscope
Optical Engineering, 1995We present the design, construction, and characterization of a spherical-coordinate scanning, reflection-mode confocal laser microscope (SCSLM). Two system designs are presented, namely a conventional bulk optics object-scanning design and a preliminary optical-fiber-based two-axis optics/one-axis object scanning version.
Tilemachos Doukoglou, Ian W. Hunter
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Variable-ambient scanning stage for a laser scanning confocal microscope
Review of Scientific Instruments, 2003A variable-ambient scanning stage for a laser scanning confocal microscope was designed and tested. The stage allows for facile observation of both thin films and single molecule samples under inert conditions. High precision images have been acquired up to 174 μm2 using a single piezoceramic tube.
D. J. Sirbuly +4 more
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Three-Dimensional Microscopy Using a Confocal Laser Scanning Microscope
Optics Letters, 1985In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen. The recorded digital images constitute a three-dimensional raster covering a volume of the specimen.
Reiner Lenz, K Carlsson
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Identification of Surface Topography Scanned by Laser Scanning Confocal Microscope
Applied Mechanics and Materials, 2014Topography and surface roughness is defined as a set of surface textures formed due to used machining or other production technology. It is a key factor mainly for dynamically stressed components. Greater surface roughness negatively affects the fatigue strength of component or its wear resistance.The paper deals with the visualization and processing ...
Tomas Bezak +5 more
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A Confocal Laser Scanning Microscope Investigation of the Epiphany Obturation System
Journal of Endodontics, 2007The purpose of this study was to assess the sealer-dentin interface and compare the percentage and average depth of dentinal tubule sealer penetration in the coronal, middle and apical thirds of teeth obturated with the Epiphany Obturation System using 10x and 40x confocal laser scanning microscopy.
Saman R, Gharib +4 more
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A UV laser-scanning confocal microscope for the measurement of intracellular Ca2+
Cell Calcium, 1994Modifications to the optics of a conventional confocal laser-scanning microscope were made to allow imaging intracellular Ca(2+)-dependent fluorescence with a UV laser (351 or 364 nm). Modifications included: (1) a chromatic compensation lens in the laser path; (2) the design of a practically achromatic relay lens; (3) a longer tube length for the ...
K, Kuba, S Y, Hua, T, Hayashi
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The Intermediate Optical System of Laser-scanning Confocal Microscopes
1990This text explains some of the basics of intermediate optical systems in confocal microscopes. Important microscope components are discussed in more detail in other chapters: lasers (Chapter 5) and other light sources (Chapter 6), objective lenses (Chapter 7), special optical elements (Chapter 3), fibers (Chapter 26), and detectors (Chapter 12 ...
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A video rate confocal laser scanning microscope
Optical Society of America Annual Meeting, 1992A video rate confocal laser scanning microscope (CLSM) for biological applications has been developed for manufacture. This microscope is used for viewing thin "optical sections" of a sample by rejecting the signal from out-of-focus planes. The microscope consists of a multiline argon ion laser, a galvanometer and acousto-optic deflector (AOD) for ...
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Ultrastructural and confocal laser scanning microscopic examination of TUNEL‐positive cells
The Journal of Pathology, 1997TdT-mediated dUTP-biotin nick end labelling (TUNEL) has been widely used for detecting cells with DNA fragmentation or apoptotic cells. However, since the concept of apoptosis is based on cellular ultrastructure, it is important to identify the morphological features of TUNEL-positive cells.
K, Kimura +9 more
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