Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery [PDF]
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Attila Bokros +10 more
core +2 more sources
Aiming to detail data obtained through brightfield microscopy (BM) on reproductive, excretory and digestive system, specimens of Schistosoma mansoni eight weeks old, were recovered from SW mice, stained with Langeron's carmine and analyzed under a ...
José Roberto Machado-Silva +3 more
doaj +1 more source
Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe +295 more
core +2 more sources
Exploring non-soluble particles in hailstones through innovative confocal laser and scanning electron microscopy techniques [PDF]
This paper introduces an innovative microscopy analysis methodology to preserve in situ non-soluble particles within hailstones using a protective porous plastic coating, overcoming previous limitations related to melting the hailstone sample. The method
A. C. Bernal Ayala +6 more
doaj +1 more source
Cholesterol-directed nanoparticle assemblies based on single amino acid peptide mutations activate cellular uptake and decrease tumor volume. [PDF]
Peptide drugs have been difficult to translate into effective therapies due to their low in vivo stability. Here, we report a strategy to develop peptide-based therapeutic nanoparticles by screening a peptide library differing by single-site amino acid ...
Landry, Markita P +4 more
core +1 more source
Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM).
Hiroshi Nakaguchi +13 more
doaj +1 more source
Determination of biosorption mechanism in biomass of agave, using spectroscopic and microscopic techniques for the purification of contaminated water [PDF]
[Abstract] Lead (Pb2+) and copper (Cu2+) are polluting metals due to their toxicity; however, the extraction of these metals is essential for economic development, so it is important to look for efficient and low-cost alternatives that can remove heavy ...
Barriada, José Luis +6 more
core +1 more source
The determination of gene expression patterns in three dimensions with cellular resolution is an important goal in developmental biology. However, the most sensitive, efficient, and widely used staining technique for whole-mount in situ hybridization ...
Gáspár Jékely, Detlev Arendt
doaj +1 more source
Antimicrobial activity of biogenically produced spherical Se-nanomaterials embedded in organic material against Pseudomonas aeruginosa and Staphylococcus aureus strains on hydroxyapatite-coated surfaces [PDF]
In an effort to prevent the formation of pathogenic biofilms on hydroxyapatite (HA)-based clinical devices and surfaces, we present a study evaluating the antimicrobial efficacy of Spherical biogenic Se-Nanostructures Embedded in Organic material (Bio Se-
Demeter M. +7 more
core +1 more source
The use of inhibitors to study endocytic pathways of gene carriers: optimization and pitfalls [PDF]
Nonviral gene complexes can enter mammalian cells through different endocytic pathways. For efficient optimization of the gene carrier it is important to profile its cellular uptake, because this largely determines its intracellular processing and ...
Dries Vercauteren +57 more
core +3 more sources

