Results 31 to 40 of about 2,617,420 (275)

Cellular resolution expression profiling using confocal detection of NBT/BCIP precipitate by reflection microscopy

BioTechniques, 2007
The determination of gene expression patterns in three dimensions with cellular resolution is an important goal in developmental biology. However, the most sensitive, efficient, and widely used staining technique for whole-mount in situ hybridization ...
Gáspár Jékely, Detlev Arendt
doaj   +1 more source

Research progress on the methods of observation and analysis of dental bonding interfaces

口腔疾病防治, 2021
Dental bonding technology and materials have been used widely in dentistry because of their excellent properties. The development of novel bonding technology and materials is constantly being performed to improve the effect of dental bonding restorations.
MA Chaoqun, LIU Meng, QIU Jun, LIAO Zixuan, ZHOU Wei, CHEN Jihua   +5 more
doaj   +1 more source

ATP‐Mediated Bidirectional Communication Between Natural and Artificial Cells for Controlled Bioreactions

Advanced Functional Materials, EarlyView.
Establishment of bidirectional communication behavior between natural and artificial cells. The first type of communication from natural dendritic cells to artificial polymeric cells was achieved via ATP release after cell lysis. The released ATP was taken up via gated UV‐controlled permeability increase of the polymeric membrane for performing ...
Ilona Kalte, Tsvetomir Ivanov, Leonhard Seufert, Shoupeng Cao, Lucas Caire da Silva, Volker Mailänder, Katharina Landfester   +6 more
wiley   +1 more source

In Situ Interfacial Assembly of Dynamic Semi‐Interpenetrating Biopolymer Networks for Robust and Mechanically Adaptive Droplet Interfaces

Advanced Functional Materials, EarlyView.
A dynamic interfacial semi‐interpenetrating polymer network is constructed by sequential assembly of β‐sheet‐rich SF and CS‐RL. The SF framework provides mechanical strength, while dynamically associated CS‐RL enables local rearrangement, allowing droplets to rapidly reconfigure without loss of interfacial integrity.
Yaping Zhang, Yujie Liu, Yikai Xu, Xunda Feng, Ling Zhang, Hongbo Zeng, Hao Liu, Xiaohui Mao   +7 more
wiley   +1 more source

Bacteria (E. coli) take up ultrasmall gold nanoparticles (2 nm) as shown by different optical microscopic techniques (CLSM, SIM, STORM)

Nano Select, 2022
The uptake of fluorescently labeled ultrasmall gold nanoparticles (2 nm) by Gram‐negative Escherichia coli bacteria occurs within 1–3 hours. This was demonstrated by confocal laser scanning microscopy (CLSM), structured illumination microscopy (SIM ...
Nataniel Białas, Viktoriya Sokolova, Selina Beatrice van derMeer, Torben Knuschke, Tatjana Ruks, Kai Klein, Astrid M. Westendorf, Matthias Epple   +7 more
doaj   +1 more source

The binding of cellulase variants to dislocations: a semi-quantitative analysis based on CLSM (confocal laser scanning microscopy) images [PDF]

AMB Express, 2015
Binding of enzymes to the substrate is the first step in enzymatic hydrolysis of lignocellulose, a key process within biorefining. During this process elongated plant cells such as fibers and tracheids have been found to break into segments at irregular cell wall regions known as dislocations or slip planes.
Hidayat, Budi J., Weisskopf, Carmen, Felby, Claus, Johansen, Katja Salomon, Thygesen, Lisbeth Garbrecht   +4 more
openaire   +5 more sources

Application of Atomic Force (AFM), Environmental Scanning Electron (ESEM) and Confocal Laser Scanning Microscopy (CLSM) in bitumen: A review of the ageing effect

Micron, 2021
Undoubtedly bitumen's viscoelastic performance has received much attention in the literature. Especially, the oxidative ageing phenomenon of bitumen has been studied by several scholars from different physicochemical and mechanical perspectives due to its direct impact on asphalt performance.
Georgios Pipintakos, Navid Hasheminejad, Caitlin Lommaert, Anastassiya Bocharova, Johan Blom   +4 more
openaire   +3 more sources

Conjugated Porphyrin Polymer With Superior Charge Transfer for Photodynamic‐Immunomodulatory Prevention of CIED Infection in Diabetes

Advanced Healthcare Materials, EarlyView.
Under NIR irradiation, PTCPP generates ROS to eliminate MRSA biofilms and promote M1 macrophage polarization for phagocytosis. After bacterial clearance, the material is phagocytosed by macrophages, shifting their polarization to M2 phenotype via metabolic reprogramming, which synergistically enhances anti‐infection therapy and wound healing.
Honghao Ma, Jinguo Xu, Rongrong Gu, Chuanhui Dong, Te Chen, Yi Mu, Jing Zhu, Yating Wang, Wen Wang, Yingying Zhang, Hongyu Ma, Xiaochen Wang, Sheng Ye, Cheng Cheng   +13 more
wiley   +1 more source

Three-Dimensional Imaging by Confocal Laser Scanning Microscopy(CLSM).

Journal of Advanced Science, 2001
The recent development of optical microscopy has brought about a more refined and sophisticated apparatus, such as a confocal laser scanning microscopy (CLSM), which was originally described by Minsky (1957), and ever since has been applied to the field of medical biology. In early experiments, only fluorescent signals were detectable by CLSM. However,
openaire   +2 more sources

Electrospun Core–Shell Bioadhesive Nanofiber Sheets Enable Durable Pulmonary Air Leak Sealing Under Wet and Dynamic Conditions

Advanced Healthcare Materials, EarlyView.
A core–shell electrospun nanofiber sheet provides strong adhesion and durable sealing on the wet and dynamic lung surface. The engineered fibrous architecture maintains airtight integrity and offers a potential alternative to fibrin sealants for pulmonary air leak management in thoracic surgery.
Tomohito Okubo, Kouji Hara, Naoyuki Hatayama, Kota Takahashi, Eriko Yamazoe, Takaaki Ito, Takao Takeuchi, Yoko Koide, Mayumi Yamada, Munekazu Naito, Kohei Tahara   +10 more
wiley   +1 more source