Results 181 to 190 of about 663,489 (316)

Reply to: an assessment of the cytochrome P450 2-hydroxyisoflavanone synthase (2-HIS) crystal structure. [PDF]

Commun Biol
Wang X, Pan H, Sagurthi SR, Dixon RA.
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Exploring the conformational dynamics and thermodynamics of EGFR S768I and G719X + S768I mutations in non-small cell lung cancer: An in silico approaches

Open Life Sciences, 2023
Wang Jun-Ling, Liu Ming-Sheng, Fu Yu-Dong, Kan Qiang-Bo, Li Chun-Yan, Ma Rong, Fang Zhe-Wei, Liu Hong-Xia, Li Meng-Xian, Lv Jia-Ling, Sang Peng, Zhang Chao, Li Hong-Wei   +12 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Condensates as Conformation Editors of Disordered Client Proteins. [PDF]

J Am Chem Soc
Wang L, Marrink SJ.
europepmc   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Structural insights into urocanate reductase using room-temperature X-ray crystallography. [PDF]

Acta Crystallogr D Struct Biol
Aggarwal S, Gurav N, Oksanen E, Lindkvist-Petersson K, Venskutonytė R.   +4 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates

, 2010
Enzymatic catalysis of biochemical reactions is essential to all living systems. The "lock and key" and "induced fit" models were early contributions to our understanding of the mechanisms involved in the reaction between an enzyme and its substrate ...
He, Rong-Qiao, Zhang, Zi-Jian, Pan, Rong, Tian, Wei-Xi, Zhang, Xue-Jing, Zhou, Yuan   +5 more
core  

Multimerization interactions between protein-inspired single-chain random heteropolymers. [PDF]

PLoS One
Hilburg SL, Jin T, Ganesh P, Alexander-Katz A.   +3 more
europepmc   +1 more source