Results 31 to 40 of about 323,565 (233)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Longitudinal Assessment of Biomarkers in ALS: Discriminative Biomarkers for Disease Progression and Survival

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To assess the association and discriminative performance of serum biomarkers with clinical disease progression and survival in patients with amyotrophic lateral sclerosis (ALS). Methods This retrospective study, conducted at Houston Methodist Hospital, Houston, TX, used longitudinal serum samples collected between January 2018 and ...
David R. Beers, Yueh‐Yun Lin, Jason R. Thonhoff, Aaron D. Thome, Alireza Faridar, Weihua Zhao, Shixiang Wen, Stanley H. Appel   +7 more
wiley   +1 more source

Effects of subtended and vertex angles on the vibration characteristics of conical shell panels: a conical coordinate approach

, 1999
This paper presents a computational investigation on the effects of subtended and vertex angles on the free vibration characteristics of open conical shell panels.
Lim, C. W., Kitipornchai, S.
core   +1 more source

Current Status and Challenges in Data Collection for Aerospace Coatings Deposited by Plasma Spraying

Advanced Engineering Materials, EarlyView.
An innovative approach has been integrated into the GRENAT project to optimize plasma spraying and coating performance. Raw materials are accelerated and melted in the plasma generated by torches, creating coatings. Monitoring sensors collect process data which are combined with ex situ characterization data.
Lila Randriamananjara, Vincent Rat, Quentin Dubreuil, Patrice Valorge, Simon Goutier, Alain Denoirjean, Olivier Messe, Céline Ruelle, Jean‐Philippe Poli   +8 more
wiley   +1 more source

Experimental and Numerical Study on Damage Characteristics of Web Frame Structure Under Conical Impact

Journal of Marine Science and Engineering
This paper investigates the dynamic performance of web frame structures under the impact of a conical hammer head. Compared with existing research on flat plates and stiffened panels, web frame structures exhibit significant differences in load-bearing ...
Zhengjie Li, Caixia Jiang, Gaofei Wang, Nan Zhao, Yue Lu, Kun Liu   +5 more
doaj   +1 more source

Thermo-mechanical behaviour of TRIP 1000 steel sheets subjected to low velocity perforation by conical projectiles at different temperatures [PDF]

, 2010
This paper presents and analyzes the behaviour of TRIP 1000 steel sheets subjected to low velocity perforation by conical projectiles. The relevance of this material resides in the potential transformation of retained austenite to martensite during ...
Rodríguez-Martínez, José Antonio, Pedroche Arias, David, Arias Hernández, Ángel, Pesci, R., Zaera Polo, Ramón Eulalio, ARIAS, Angel, RUSINEK, Alexis, Rodríguez-Martínez, José A., PESCI, Raphaël, Arias Pedroche, David, PEDROCHE, David Arias, RODRIGUEZ MARTINEZ, José Antonio, ZAERA, Ramon   +12 more
core   +1 more source

Enhancement of the Through‐Thickness Electrical Conductivity of Carbon‐Fiber‐Reinforced Plastics Using Large Graphite Particles

Advanced Engineering Materials, EarlyView.
To enhance through‐thickness conductivity without sacrificing impregnation, large spherical graphite particles are intentionally employed in a low‐viscosity resin. Unlike finer conductive fillers, these particles remain outside the fiber bundles and accumulate in resin‐rich interlaminar regions during molding.
Keito Hosoe, Yu Zhou, Shintaro Kamiyama, Takao Okada, Tomohiro Yokozeki, Ryoma Tokonami, Tatsuhiro Takahashi   +6 more
wiley   +1 more source

Improved impact performance of marine sandwich panels using through-thickness reinforcement: Experimental results [PDF]

, 2010
This paper presents results from a test developed to simulate the water impact (slamming) loading of sandwich boat structures. A weighted elastomer ball is dropped from increasing heights onto rigidly supported panels until damage is detected. Results
Davies, Peter, Partridge, I.K, Cartie, D. D. R., Partridge, I. K., Cartié, D., Baral, N., Davies, P., Cartié, Denis D. R., Partridge, Ivana K., Baley, C.   +9 more
core   +1 more source

Embedded Ferroelectric Nanoclusters Can Drive Polarization Reversal in a Non‐Ferroelectric Polar Film via the Proximity Effect

Advanced Functional Materials, EarlyView.
Ferroelectric nanoclusters create local internal fields in a normally non‐switchable polar film because of polarization mismatch at their interfaces. That field opposes polarization in the regions with larger polarization and reinforces polarization in the regions with smaller polarization.
Anna N. Morozovska, Eugene A. Eliseev, Sergei V. Kalinin, Long‐Qing Chen, Dean R. Evans, Venkatraman Gopalan   +5 more
wiley   +1 more source