Results 191 to 200 of about 2,280,486 (356)

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

Current trends in single‐cell RNA sequencing applications in diabetes mellitus

FEBS Open Bio, EarlyView.
Single‐cell RNA sequencing is a powerful approach to decipher the cellular and molecular landscape at a single‐cell resolution. The rapid development of this technology has led to a wide range of applications, including the detection of cellular and molecular mechanisms and the identification and introduction of novel potential diagnostic and ...
Seyed Sajjad Zadian, Khodakaram Jahanbin, Shekoofeh Nikooei, Marzieh Rostaminejad, Arezoo Rahimi, Pedram Abdizadeh, Behnam Alipoor   +6 more
wiley   +1 more source

Mining LDA topics on construction engineering change risks based on graded evidence. [PDF]

PLoS One
Jin L, Li C, Zhu Z, Zou S, Sun X.
europepmc   +1 more source

Applications of Polymers in Civil Engineering and Construction Works

, 1973
Yoshihiko Ohama
openalex   +2 more sources

Construction and biological characterization of an HB-GAM/FGF-1 chimera for vascular tissue engineering [PDF]

, 2001
Lian Xue, Apostolos K. Tassiopoulos, Susanne K. Woloson, Daniel Stanton, Christine Sullivan, Brian Hampton, Wilson H. Burgess, Howard P. Greisler   +7 more
openalex   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

Building Files & Blueprints [PDF]

, 2017
Construction & Engineering Office
core   +1 more source

Assessing the impact of mega-city construction engineering on urban livability: an explorative study of Yan'an. [PDF]

Front Public Health
Liu L, Zhang L, Guo Y, Ren K.
europepmc   +1 more source

Prospects of Construction Machines(<Special Issue>Future Development of Mechanical Engineering and Industries)

, 1979
Kan-ichi Uchida
openalex   +2 more sources

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source