SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Quantifying resilience potentials in construction: pilot evaluation of the resilience assessment grid. [PDF]
Front Public HealthTierra-Arévalo JM +3 more
europepmc +1 more source
Naked cuticle is essential for Drosophila wing development beyond Wingless signaling
FEBS Open Bio, EarlyView.Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley +1 more source
A prediction method for long-term surface subsidence considering the mining-induced stratum creep effect and its application. [PDF]
Sci RepLiang Z, Yang X, Liu Y, Zhang B.
europepmc +1 more source
The outlook of blockchain technology for construction engineering management
, 2017 Jun Wang, Peng Wu, Xiangyu Wang, W. Shou
semanticscholar +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Correction: Construction of a homologous series of metal nanoclusters and implications for structure-activity correlations. [PDF]
Chem SciLi Q +5 more
europepmc +1 more source
A Guideline for the Development of a Scenario-Based Senior Capstone Course for Construction Engineering and Management Students [PDF]
Namhun Lee, Seong Jin Kim, Jiyong Choi
openalex +1 more source