Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Analysis of thermal wave scattering and temperature distribution in sub-surface, defects of gradient construction materials. [PDF]
Yang X +8 more
europepmc +1 more source
Intelligent Design of Construction Materials: A Comparative Study of AI Approaches for Predicting the Strength of Concrete with Blast Furnace Slag. [PDF]
Wu X, Zhu F, Zhou M, Sabri MMS, Huang J.
europepmc +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
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Metabolic Insights Into Microbially Induced Calcite Formation by Bacillaceae for Application in Bio-Based Construction Materials. [PDF]
Seidel M +9 more
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Effects of Mellowing Practice on the Strength and Swelling Properties of Road Construction Materials: Case of Sulphate-Bearing Clay Soils Stabilised with Lime-Silica Fume Blended Binder. [PDF]
Al-Waked Q +4 more
europepmc +1 more source
Overview of molecular signatures of senescence and associated resources: pros and cons
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas +6 more
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A Comprehensive Accounting of Construction Materials in Belt and Road Initiative Projects. [PDF]
Hou L +7 more
europepmc +1 more source
Characterization data of reference materials used for phase II of the priority program DFG SPP 2005 "Opus Fluidum Futurum - Rheology of reactive, multiscale, multiphase construction materials". [PDF]
Pott U +27 more
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Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source

