Results 51 to 60 of about 269,665 (298)

AAA+ protein unfoldases—the Moirai of the proteome

open access: yesFEBS Letters, EarlyView.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Effects of Internal Force Redistribution on the Limit States of Continuous Beams with GFRP Reinforcement

open access: yesApplied Sciences, 2020
Fiber-reinforced polymers (FRP) are commonly used as internal reinforcement in RC structures in aggressive environments. The design of concrete elements reinforced with FRP bars is usually ruled by serviceability criteria rather than the ultimate limit ...
Nikola Baša   +3 more
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Teleportation of continuous variable polarisation states [PDF]

open access: yes, 2003
This paper discusses methods for the optical teleportation of continuous variable polarisation states. We show that using two pairs of entangled beams, generated using four squeezed beams, perfect teleportation of optical polarisation states can be ...
A. Dolińska   +22 more
core   +2 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Experimental investigations of flexural and shear behavior of continuous prestressed HSC beams under repeated loads

open access: yesMeasurement: Sensors, 2023
The results of an experimental study into the flexural and shear conduct of continuous two span unbonded posttensioned high strength concrete beams subjected to static and a limited number of repeated loading cycles are presented in this paper.
Mustafa B. Dawood   +1 more
doaj   +1 more source

Generation of Generalized-Gauss Laser Beams via a Spatial Light Modulator [PDF]

open access: yes, 2018
Generalized-Gauss laser beams can be described as a continuous transition between the well-known Hermite-Gauss (HG) and Laguerre-Gauss (LG) laser beams.
Conry Young, Jessica P, Dix, Samantha
core   +3 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Vibrational Modal Frequencies and Shapes of Two-Span Continuous Timber Flooring Systems [PDF]

open access: yes, 2019
Based on classic vibrational bending theory on beams, this paper provides comprehensive analytical formulae for dynamic characteristics of two equal span continuous timber flooring systems, including frequency equations, modal frequencies, and modal ...
Kilpatrick, Tony, Zhang, Binsheng
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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