Results 201 to 210 of about 7,519 (258)

Minimizing Off‐Target Effects of CRISPR‐Cas9 With Optimized sgRNA: Evaluation of Efficiency and Specificity in the Tumor Protein 53 (TP53) Region

Biotechnology and Bioengineering, EarlyView.
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) – Cas9‐based genome editing has emerged as a widely used tool across various disciplines, ranging from molecular biology to gene therapy. This revolutionary technology, which enables precise gene editing, represents a significant advancement in biotechnology, opening new frontiers for ...
Ali Mertcan Köse, Monia Ranalli, Drenka Trivanovic, Irina Maslovaric   +3 more
wiley   +1 more source

Irrigation Challenges, Water Access, and Adaptation Strategies in Smallholder Farming: Evidence From Gaza Province, Mozambique. [PDF]

Glob Chall
Tandane HJ, Francisco BF, Maholele AJ, Sitoe MM.   +3 more
europepmc   +1 more source

LLM‐Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Mechanistic models of Chinese Hamster Ovary (CHO) cell cultures are essential for efficient bioprocess development and control, yet parameter estimation remains a critical challenge in model calibration. As model complexity increases, the number of parameters grows and their values become highly system‐specific due to biological variability ...
Han Bit Kim, Janghan Lee, Seo‐Yeon Kim, Jihoon Kim, Moo Sun Hong   +4 more
wiley   +1 more source

Broiler farming and antibiotic use through an agency theory lens. A case study from West Bengal, India. [PDF]

PLoS One
Hennessey M, Samanta I, Fournié G, Quaife M, Gautham M, Paleja H, Papaiyan K, Biswas R, Alarcon P.   +8 more
europepmc   +1 more source

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

The political economy of tobacco of Zimbabwe: An analysis of stakeholder perspectives. [PDF]

PLOS Glob Public Health
Bandara S, Kadungure A, Nhamo N, Nyashanu T, Chamunorwa A, Drope J, Hunt M, Adams A, Lencucha R.   +8 more
europepmc   +1 more source

High prevalence of the Mediterranean spotted fever agent Rickettsia aeschlimannii in Hyalomma marginatum ticks from Pianosa island, Italy. [PDF]

Parasit Vectors
Alba P, Tagliapietra V, Franco A, Forbicioni L, Coppola S, Capitani M, Stravino F, Onorati R, Ianzano A, Arnoldi D, De Liberato C, Battisti A, Rizzoli A.   +12 more
europepmc   +1 more source

Mechanisms for agricultural companies in enabling smallholders' integration into modern agriculture. [PDF]

Sci Rep
Chen Y, Zhang H, Lu Y, Song Z.
europepmc   +1 more source

Fairness and Contractual Performance in Vertical Linkages Within an Uncertain Environment: A Case of a Tomato Value Chain. [PDF]

Foods
Benmehaia AM, Samoggia A, Benharrat O, Benziouche SE, Ayfantopoulou G.   +4 more
europepmc   +1 more source