Results 201 to 210 of about 157,910 (293)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
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A contractor's perception on early contractor involvement
Built Environment Project and Asset Management, 2012PurposeThe purpose of this paper is to evaluate the perception of a contractor on early contractor involvement (ECI) type of construction project delivery approach in terms of its benefits and drawbacks.Design/methodology/approachA questionnaire survey was conducted on various professionals of a contractor, who had extensive experience of working in ...
Motiar Rahman, Aminu Alhassan
exaly +2 more sources
Contractor Evaluations in the Contractor Selection Process [PDF]
, 2014 The current contractor evaluation system in use within the Kentucky Transportation Cabinet is based on the contractor evaluation system developed as part of SPR 212-00 “Quality Based Prequalification of Contractors.” This system relies on average performance ratings from the completion of the Contractor’s Performance Report at the conclusion of every ...
Taylor, Timothy R. B. +4 more
openaire +2 more sources
, 2021
The process of bid evaluation is subject to indetermination, imprecision, and uncertainty in terms of their alternative-criterion decision appraisals. To address this issue, this paper develops a novel ELECTRE III-based MCGDM approach for bid evaluation,
Zhen-Song Chen +4 more
semanticscholar +1 more source
The process of bid evaluation is subject to indetermination, imprecision, and uncertainty in terms of their alternative-criterion decision appraisals. To address this issue, this paper develops a novel ELECTRE III-based MCGDM approach for bid evaluation,
Zhen-Song Chen +4 more
semanticscholar +1 more source