Results 21 to 30 of about 3,948,587 (242)
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
FEBS Letters, EarlyView.The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi +6 more
wiley +1 more source
Engineering Applications of Computational Fluid MechanicsSecondary-air systems (SASs) are critical for maintaining material integrity and optimizing thermal performance in gas turbines (GTs) and related energy equipment.
Yulong Yao +3 more
doaj +1 more source
Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions
FEBS Letters, EarlyView.The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang +4 more
wiley +1 more source
Development of composite cooling tower for 160 km/h power concentrated EMU
机车电传动, 2022 Cooling system is an important subsystem of power concentrated EMU. The cooling performance, volume, weight, auxiliary power and noise control of cooling devices are the technical bottlenecks and difficulties in product research and development, which ...
CHEN Guangtai +4 more
doaj
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
Experimental performance evaluation of a solar powered evaporative cooling system for preservation o
Journal of Thermal EngineeringLack of suitable power supplies and storage facilities are major contributors to post-harvest loss of fruits and vegetables in low- and middle-income nations.
Mfon O. UDO +14 more
doaj +1 more source
Effects of Bypass Flow Distribution on Cold Flow Characteristics of Integrated Afterburner
Energies, 2021 Integrated design is a trend in the development of afterburners, and the distribution of cold flow is directly related to their flow field characteristics, combustion organization, and the cooling effect of components.
Xiangzhong Jia +4 more
doaj +1 more source
FEBS Letters, EarlyView.Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell +2 more
wiley +1 more source
Nanjing Hangkong Hangtian Daxue XuebaoThis study proposes a novel multi‑spectral light-field temperature measurement method that integrates multi‑spectral radiation thermometry and light‑field imaging technology. Through the coupled design of an optical filter array and a light‑field camera，
FANG Hua +9 more
doaj +1 more source