Results 71 to 80 of about 1,288,708 (302)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Control-Oriented Modeling of the Cooling Process of a PEMFC-Based $\mu$ -CHP System

IEEE Access, 2019
Micro-combined heat and power systems (μ-CHP) based on proton exchange membrane fuel cell stacks (PEMFC) are capable of supplying electricity and heat for the residential housing sector with a high energy efficiency and a low level of CO2 ...
Santiago Navarro Gimenez, Juan Manuel Herrero Dura, Francesc Xavier Blasco Ferragud, Raul Simarro Fernandez   +3 more
doaj   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

TC48: A low-cost 48 V integrated drive for mild hybrid electric vehicles

The Journal of Engineering, 2019
The TC48 drive is a low-cost, low-voltage integrated motor-drive solution suitable for mild hybrid electric vehicles, off-highway traction, and any application where a compact, highly torque-dense machine is required.
Dave Winterborne, Muez Shiref, Stuart Snow, Volker Pickert   +3 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Thermal Characteristics Investigation of the Internal Combustion Engine Cooling-Combustion System Using Thermal Boundary Dynamic Coupling Method and Experimental Verification

Energies, 2018
The engine cooling system must be able to match up with the stable operating conditions so as to guarantee the engine performance. On the working cycle level, however, the dynamic thermo-state of engines has not been considered in the cooling strategy ...
Junhong Zhang, Zhexuan Xu, Jiewei Lin, Zefeng Lin, Jingchao Wang, Tianshu Xu   +5 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Development of TGA18 Water-Cooling Traction Converter for Diesel Locomotive

机车电传动, 2012
Main circuit, electric technic parameters and overall structure of TGA18 water-cooling traction converter for high-power diesel locomotive were introduced, focusing on converter-device selection and structure of the cooling system.
ZHANG Yi, RAO Pei-nan, CHEN Yan-ping
doaj  

Ground state cooling of a nanomechanical resonator in the weak-confinement regime via quantum interference

, 2009
Ground state cooling of a nanomechanical resonator coupled to a superconducting flux qubit is discussed. We show that by inducing quantum interference to cancel detrimental carrier excitations, ground state cooling becomes possible in the weak ...
Evers, J., Xia, K.
core   +1 more source

SPG4 and Dementia: Expanding the Clinical Spectrum

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Hereditary spastic paraplegia (HSP) is a group of disorders characterized by progressive spasticity and lower limb weakness, with mutations in SPG4/SPAST being the most common cause. Detailed studies and clinical and molecular comparisons across different populations are missing.
Emanuele Panza, Arun Meyyazhagan, Eliseo Picchi, Gustavo Ribas, Ingrid Faber, Ryosuke Miyamoto, Preethi Basavaraju, Paolo Eusebi, Haripriya Kuchi Bhotla, Mario Stasi, Fabrizio Gaudiello, Francesco Patti, Filippo Maria Santorelli, Marcondes Cavalcante França Jr, José Luiz Pedroso, Orlando Graziani Povoas Barsottini, Hélio Afonso Ghizoni Teive, Peter Henry St George‐Hyslop, Toshitaka Kawarai, Antonio Orlacchio   +19 more
wiley   +1 more source