Results 151 to 160 of about 9,837,393 (260)

SPHINX31 acts as a SRPK1 inhibitor targeting the ATR/DNA‐PKcs/CHK1 replicative checkpoint to inhibit cell growth in non‐small cell lung cancer

Molecular Oncology, EarlyView.
The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim, Aurelie Genoux, Nadiia Zubchuk, Haoyang Zheng, Perla Salameh, Theo Ziegelmeyer, Fabien Dalonneau, Margot Rouchette, Christiane Oddou, Simona Miron, Helene Polveche, Adiilah Mamode Cassim, Carmen Garrido, Eleni Nikolakaki, Didier Auboeuf, Tao Jia, Sophie Zinn‐Justin, Beatrice Eymin   +17 more
wiley   +1 more source

A hybrid system enables plasmid copy number control in yeast. [PDF]

Nat Commun
Li A, Zhao Q, Yang Z, Cheng J, Xu D, Zhang Y, He T, Zhuo B, Zhao X, Rao X, Wang H, Chen L, Luo Z.   +12 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

CNV-Finder: streamlining copy number variation discovery. [PDF]

Bioinform Adv
Kuznetsov N, Daida K, Makarious MB, Al-Mubarak B, Atterling Brolin K, Malik L, Kouam C, Baker B, Real R, Step K, Lange LM, Wu L, Ostrozovicova M, Andersh KM, Kung PJ, Mecheri Y, Tay YW, Soundous Malek B, Al Tassan N, Periñan MT, Hong S, Koretsky MJ, Sargeant L, Levine K, Blauwendraat C, Billingsley KJ, Bandres-Ciga S, Leonard HL, Bardien S, Morris HR, Singleton AB, Nalls MA, Vitale D.   +32 more
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Complex landscape of somatic copy number alterations in head and neck paragangliomas. [PDF]

Front Endocrinol (Lausanne)
Pavlov VS, Fedorova MS, Elnukaev TSM, Kalinin DV, Pudova EA, Katunina IV, Guvatova ZG, Kobelyatskaya AA, Kaprin AD, Kudryavtseva AV, Snezhkina AV.   +10 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Demo: DNA Copy Number Analysis

, 2017
Week 3: DNA and Big Data 3.2 DNA Copy Number DNA Copy Number DemoDemystifying Biomedical Big Data BIOX-201 ...
Haddad, Bassem, Gusev, Yuriy, McGarvey, Peter   +2 more
core  

Pathways of rDNA copy number homeostasis in Schizosaccharomyces pombe. [PDF]

G3 (Bethesda)
Jones CE, Yuan JP, Forsburg SL.
europepmc   +1 more source