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Engineering Corynebacterium glutamicum for the production of pyruvate
Applied Microbiology and Biotechnology, 2012A Corynebacterium glutamicum strain with inactivated pyruvate dehydrogenase complex and a deletion of the gene encoding the pyruvate:quinone oxidoreductase produces about 19 mM L: -valine, 28 mM L: -alanine and about 55 mM pyruvate from 150 mM glucose. Based on this double mutant C. glutamicum △aceE △pqo, we engineered C.
Stefan, Wieschalka +2 more
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Synthetic promoter libraries for Corynebacterium glutamicum
Applied Microbiology and Biotechnology, 2014The ability to modulate gene expression is an important genetic tool in systems biology and biotechnology. Here, we demonstrate that a previously published easy and fast PCR-based method for modulating gene expression in lactic acid bacteria is also applicable to Corynebacterium glutamicum.
Jakob Vang, Rytter +5 more
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Sigma factors and promoters in Corynebacterium glutamicum
Journal of Biotechnology, 2011The Corynebacterium glutamicum genome codes for 7 sigma subunits (factors) of RNA polymerase (RNAP): primary sigma factor SigA (σ(A)), primary-like SigB and 5 other alternative sigma factors (SigC, SigD, SigE, SigH and SigM). Each sigma factor is responsible for recognizing promoters of genes belonging to a regulon (sigmulon) involved in specific ...
Miroslav, Pátek, Jan, Nešvera
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Putrescine production by engineered Corynebacterium glutamicum
Applied Microbiology and Biotechnology, 2010Here, we report the engineering of the industrially relevant Corynebacterium glutamicum for putrescine production. C. glutamicum grew well in the presence of up to 500 mM of putrescine. A reduction of the growth rate by 34% and of biomass formation by 39% was observed at 750 mM of putrescine. C.
Schneider, Jens, Wendisch, Volker F.
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Protein secretion inCorynebacterium glutamicum
Critical Reviews in Biotechnology, 2016Corynebacterium glutamicum, a Gram-positive bacterium, has been widely used for the industrial production of amino acids, such as glutamate and lysine, for decades. Due to several characteristics - its ability to secrete properly folded and functional target proteins into culture broth, its low levels of endogenous extracellular proteins and its lack ...
Xiuxia, Liu +5 more
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Transformation of spheroplasts and protoplasts of Corynebacterium glutamicum
Applied Microbiology and Biotechnology, 1988Spheroplasts were prepared from Corynebacterium glutamicum ATCC13032 by growing cells in the presence of glycine followed by digestion with lysozyme. Using pUL330 a spheroplast transformation system was established routinely yielding 103 to 104 transformants per μg of plasmid DNA.
THIERBACH, G +2 more
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A transketolase mutant of Corynebacterium glutamicum
Applied Microbiology and Biotechnology, 1998A transketolase mutant was first isolated from Corynebacterium glutamicum, an organism of industrial importance. The mutant strain exhibited an absolute requirement for shikimic acid or the aromatic amino acids and vitamins for growth, and also failed to grow on ribose or gluconic acid as sole carbon source, even with the aromatic supplement.
M. Ikeda, K. Okamoto, R. Katsumata
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Recent progress in production of amino acid‐derived chemicals using Corynebacterium glutamicum
World Journal of Microbiology & Biotechnology, 2021Y. Tsuge, Hiroki Matsuzawa
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Handbook of Corynebacterium glutamicum
2005Part I: History A Short History of the Birth of the Amino Acid Industry in Japan, S. Kinoshita Part II: Taxonomy Corynebacterium Taxonomy, W. Liebl Part III: Genome, Plasmids and Gene Expression The Genomes of Amino Acid-producing Corynebacteria, J. Kalinowski Native Plasmids of Amino Acid-producing Corynebacteria, A.
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Analysis of Corynebacterium glutamicum Promoters and Their Applications
2012Promoters are DNA sequences which function as regulatory signals of transcription initiation catalyzed by RNA polymerase. Since promoters substantially influence levels of gene expression, they have become powerful tools in metabolic engineering. Methods for their localization used in Corynebacterium glutamicum and techniques for the analysis of their ...
Jan, Nešvera +2 more
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