Results 241 to 250 of about 1,039,545 (289)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source
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Critical Discourse Analysis

2020
Critical discourse analysis (CDA) is a social scientific theory and method for analyzing and critiquing the use of language and its contribution to forming and sustaining social practice and for analysis of how language can contribute to reproducing or transforming social problems.
Joan Cutting, Kenneth Fordyce
  +5 more sources

Critical Discourse Analysis

2013
AbstractCritical Discourse Analysis (CDA) has entered the mainstream of linguistic and social science research with a strong transdisciplinary orientation and social engagement. This chapter introduces six variants of CDA: (1) Fairclough’s approach, which is strongly social theoretically embedded and informed by systemic functional linguistics; (2) van
  +8 more sources

Critical discourse analysis

2022
This book provides a comprehensive account of the discipline of Critical Discourse Analysis (CDA) and demonstrates multiple linguistic methods through which it exposes and demystifies ideologies that are present in institutional discourse. The book enables readers to critique the complexities of the relationship between language and power to expose the
  +5 more sources

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