Results 61 to 70 of about 6,620,238 (311)

Human colon organoid differentiation from induced pluripotent stem cells using an improved method

FEBS Letters, Volume 599, Issue 6, Page 912-924, March 2025.
We compared established protocols to differentiate induced pluripotent stem cells into colon organoids and found that activation of bone morphogenetic protein 2 signaling induced gene expression of colon‐specific markers. Adenovirus‐mediated transduction of the transcription factors HOXD13 or SATB2 further enhanced colonic gene expression, suggesting ...
I‐Ting Lee, Yu Takahashi, Takashi Sasaki, Yoshio Yamauchi, Ryuichiro Sato   +4 more
wiley   +1 more source

Elucidation of interface interactions between a dehydratase domain and an acyl carrier protein in cremimycin polyketide synthase

FEBS Letters, Volume 599, Issue 8, Page 1159-1168, April 2025.
In modular polyketide synthases, the dehydratase (DH) domain catalyzes the dehydration reaction of the β‐hydroxyacyl unit attached to the cognate acyl carrier protein (ACP) domain. However, it is unclear how DH interacts with ACP during the reaction. In this study, we identified DH–ACP interface residues, providing the first detailed insights into DH ...
Kaede Kotagiri, Haruka Tachibana, Daisuke Kawasaki, Taichi Chisuga, Toma Kashima, Shinya Fushinobu, Fumitaka Kudo, Tadashi Eguchi, Akimasa Miyanaga   +8 more
wiley   +1 more source

The apo LETM1 F‐EF‐hand adopts a closed conformation that underlies a multi‐modal sensory role in mitochondria

FEBS Letters, Volume 599, Issue 7, Page 971-988, April 2025.
We present the first solution structure of the Ca2+‐depleted LETM1 F‐EF‐hand through a D676A/N678A Ca2+ binding‐deficient mutant, revealing a closed hydrophobic cleft caused by a unique F1‐helix pivot. The apo LETM1 F‐EF‐hand exhibits regiospecific hot and cold unfolding, sensitivity to physiological pH changes and potential for promiscuous heterotypic
Qi‐Tong Lin, Danielle M. Colussi, Peter B. Stathopulos   +2 more
wiley   +1 more source

Making tau amyloid models in vitro: a crucial and underestimated challenge

FEBS Letters, EarlyView.
This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley   +1 more source

Overview of Cross-Chain Identity Authentication Based on DID [PDF]

Jisuanji kexue yu tansuo
With the emergence of concepts such as metaverse and Web3.0, blockchain plays a very important role in many fields. Cross-chain technology is an important technical means to achieve inter-chain interconnection and value transfer.
BAI Yirui, TIAN Ning, LEI Hong, LIU Xuefeng, LU Xiang, ZHOU Yong
doaj   +1 more source

Extending Atomic Cross-Chain Swaps [PDF]

, 2019
Cryptocurrencies enable users to send and receive value in a trust-less manner. Unfortunately trading the associated assets usually happens on centralized exchange which becomes a trusted third party. This defeats the purpose of a trust-less system. Atomic cross-chain swaps solve this problem for exchanges of value without intermediaries.
Jean-Yves Zie, Jérémy Briffaut, Jean-Christophe Deneuville, Benjamin Nguyen   +3 more
openaire   +2 more sources

Unraveling Mycobacterium tuberculosis acid resistance and pH homeostasis mechanisms

FEBS Letters, EarlyView.
Mycobacterium tuberculosis exhibits a remarkable resilience to acid stress. In this Review, we discuss some of the molecular mechanisms and metabolic pathways used by the tubercle bacilli to adapt and resist host‐mediated acid stress. Mycobacterium tuberculosis (Mtb) is a successful pathogen that has developed a variety of strategies to survive and ...
Janïs Laudouze, Stéphane Canaan, Alexandre Gouzy, Pierre Santucci   +3 more
wiley   +1 more source

SMPTC3: Secure Multi-Party Protocol Based Trusted Cross-Chain Contracts

Mathematics
We propose an innovative approach called SMPTC3, designed specifically to enhance security and privacy in cross-chain transaction verification. This approach addresses multi-chain, multi-participant information exchange and large-scale cross-chain ...
Hanyu Mao, Tiezheng Nie, Minghe Yu, Xiaomei Dong, Xiaohua Li, Ge Yu   +5 more
doaj   +1 more source

Multi-Core and Cross-Chain Evaluation Method Based on Multi-Core Mesh Collaboration Relationship

IEEE Access, 2020
With the increasing economic globalization and the cooperation system of division of labor in the automobile industry, the multi-core network complex cooperative relationship between upstream and downstream enterprises and automobile manufacturing ...
Binyong Li, Shihao Liu, Yanqun Guo, Zeyan Du, Zhengyi Lei, Zhe Ding   +5 more
doaj   +1 more source

The solution supramolecular structure of α2 → 8 polysialic acid suggests a structural cause for its low immunogenicity

FEBS Letters, EarlyView.
α2 → 8 polysialic acid elicits poor immunogenicity. Small‐angle scattering shows a supramolecular structure with parallel‐chain binding, although in different forms at μm and mm calcium. The major histocompatibility complex requires molecular weights around 2000 Da to produce antibodies, and 2000 Da polysialic oligomers will bind in these structures ...
Kenneth A. Rubinson
wiley   +1 more source