Results 91 to 100 of about 4,667,155 (305)

The Development Of Multifunctional Protein Cross-linking Reagents Based Upon Citraconimide And Citraconic Anhydride Derivatives.

, 1986
A series of reagents have been developed which can interact with nucleophilic sulfhydryl groups on proteins by a consecutive sequence of Michael reactions to form intra- and intermolecular cross-linked structures.
Dupont, Andrea Christine
core   +3 more sources

IMMOBILIZATION OF MICROBIAL CELLS FOR THE PRODUCTION OF ORGANIC ACIDS [PDF]

Journal of Sciences, Islamic Republic of Iran, 1990
Techniques of immobilization of microbial- cells have been established by multifunctional reagents such as glutaraldehyed on a solid support. Immobilized cell reactor was designed to demonstrate stability of cross linking of whole cells for the ...
doaj  

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

Ultrafast visual detection of Streptococcus suis via HtpsC‐targeted LAMP–CRISPR–Cas12a coupled with lateral flow immunochromatography

FEBS Open Bio, EarlyView.
A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li, Miao Xin, Xiao‐wen Cui, Shi‐yin Yu, Fei Meng, Min Cao, Xiao‐hui Xiong, Yi‐chen Lu   +7 more
wiley   +1 more source

Reversibly locked thionucleobase pairs in DNA to study base flipping enzymes

Beilstein Journal of Organic Chemistry, 2014
Covalently interstrand cross-linked DNA is an interesting tool to study DNA binding proteins that locally open up the DNA duplex by flipping single bases out of the DNA helix or melting whole stretches of base pairs to perform their function.
Christine Beuck, Elmar Weinhold
doaj   +1 more source

Dactolisib outperforms Rapamycin in potentiating apoptosis induced by XPO1, Bcl‐2 and Mcl‐1 blockade in preclinical glioblastoma models

FEBS Open Bio, EarlyView.
The PAM pathway and its impact on cellular metabolism. Color‐coded key symbols represent Rapamycin (R, orange, targeting mTOR) and Dactolisib (D, green, targeting PI3K/mTOR), indicating their sites of action. Created with BioRenders.com. Glioblastoma resists therapy through interconnected survival programs.
Kai Zhao, Leonie Meyer, Madita Braun, Katharina Otte, Celine Bitter, Maite Pawlik, Hartmann Raifer, Frederik Helmprobst, Axel Pagenstecher, Hans Urban, Michael C. Burger, Michael W. Ronellenfitsch, Joachim P. Steinbach, Jarmila Jedelská, Shamim Bagheri, Behrooz H. Yousefi, Pietro Di Fazio, Markus Luster, Andreas H. Mahnken, Andreas Neubauer, Andreas Burchert, Christopher Nimsky, Jörg W. Bartsch, Tillmann Rusch   +23 more
wiley   +1 more source

Conjugation of horseradish peroxidase to staphylococcal protein A with benzoquinone, glutaraldehyde, or periodate as cross-linking reagents.

, 1981
Horseradish peroxidase was conjugated to Staphylococcal protein A by three different two-step procedures using an increasing excess of peroxidase in the second step reaction.
H Nygren, H A Hansson
core   +1 more source

Peripheral Neutrophil Activation and Extracellular Trap Formation in Amyotrophic Lateral Sclerosis

Annals of Clinical and Translational Neurology, EarlyView.
Markers of neutrophil activation are increased in plasma during ALS, and markers of NET formation associate with ALS survival. ABSTRACT Objectives Peripheral neutrophil levels in amyotrophic lateral sclerosis (ALS) inversely correlate with survival, suggesting a role for neutrophils in disease progression.
Lillia A. Baird, Haley McQuown, Jihyun Park, Samuel J. Teener, Ian F. Webber‐Davis, Andrew D. Carter, Dae‐Gyu Jang, Eva L. Feldman, Stephen A. Goutman, Benjamin J. Murdock   +9 more
wiley   +1 more source

Trioxane-based MS-cleavable cross-linking mass spectrometry for profiling multimeric interactions of cellular networks

Nature Communications
Cross-linking mass spectrometry (XL-MS) is a powerful technology for mapping protein-protein interactions (PPIs) at the systems level. While bivalent cross-links are effective for defining protein interactions and structures, multivalent cross-links ...
Clinton Yu, Eric Novitsky, Sree Ganesh Balasubramani, Xiaorong Wang, Xiyu Shen, Qin Yang, Scott Rychnovsky, Ignacia Echeverria, Lan Huang   +8 more
doaj   +1 more source

In vivo evaluation of biocompatibility and biodegradation of porcine collagen membranes

Regenerative Therapy
Introduction: Collagen-based materials differ in absorption time, biodegradation patterns, and inflammatory cell infiltration. This study aimed to evaluate the biocompatibility and biodegradation of native, differently processed, and cross-linked porcine
Seon Ae Kim, Eun Jeong Go, Bo Seung Bae, Jae Woong Jung, Mi-La Cho, Asode Ananthram Shetty, Seok Jung Kim   +6 more
doaj   +1 more source