Results 311 to 320 of about 397,014 (342)
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Chemical cross-links of proteins by using bifunctional reagents
International Journal of Biochemistry, 1984Abstract 1. 1. The chemical identification of spatial arrangements of the subunits in oligomeric proteins is exclusively achieved by the analysis of the reaction products of the protein and bifunctional reagents. 2. 2. Since the pioneer work of Hartman and Wold ( Biochemistry 6 , 2439–2448, 1967) the bifunctional reagent such as bis-imido ...
Kia-Ki Han +2 more
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A new cleavable reagent for cross-linking and reversible immobilization of proteins
Biochemical and Biophysical Research Communications, 1979Abstract We have prepared a new bifunctional reagent for the cross-linking and reversible immobilization of proteins through their amine groups. This compound, ethylene glycolyl bis(succinimidyl succinate), reacts rapidly with proteins, at pH 7 and at high dilution.
Garfield P. Royer +2 more
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A disulfide-bridge bifunctional imidoester as a reversible cross-linking reagent
Biochemical and Biophysical Research Communications, 1975Abstract A disulfide-bridged bifunctional imidoester, dimethyl 3, 3′ dithio-bispropionimidate (DTP) has been prepared and investigated as a reagent to introduce covalent cross-links in proteins that can subsequently be broken by mild reduction. Such reversible cross-links were shown to be introduced by DTP in the soluble subunit proteins aldolase and
S. J. Singer +3 more
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Bioconjugate chemistry, 2010
A novel, biocompatible, and nontoxic dermal filler using hyaluronic acid (HA) hydrogels was successfully developed for tissue augmentation applications.
J. Yeom +7 more
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A novel, biocompatible, and nontoxic dermal filler using hyaluronic acid (HA) hydrogels was successfully developed for tissue augmentation applications.
J. Yeom +7 more
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Synthesis of a Water-Soluble Protein Cross-Linking Reagent
Biological Chemistry Hoppe-Seyler, 19902,4-Difluoro-5-nitrobenzenesulfonic acid has been synthesized by the sulfonation of 2,4-difluoronitrobenzene, and precipitated with KCl as the potassium sulfonate. The structure was confirmed by chemical and spectroscopic methods (IR, 1H-NMR, 13C-NMR, 19F-NMR, UV, MS and ultimate organic analysis). Lysozyme was cross-linked with the potassium sulfonate
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The monothioacetal group, a new cleavable center for cross-linking reagents
Biochemical and Biophysical Research Communications, 1979Abstract A protein cross-linking reagent which contains a monothioacetal moiety is described. Cross-links generated using this reagent may be specifically cleaved by dilute mercuric ion at neutral pH.
F.W. Dahlquist, D.C. Muchmore
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Analytical Biochemistry, 1991
A novel, highly water-soluble, heterobifunctional cross-linking reagent, S-(2-thiopyridyl)-L-cysteine hydrazide (TPCH), was synthesized which contains a hydrazide moiety for coupling to aldehyde groups generated in the carbohydrate residues of antibodies by mild periodate oxidation, and a pyridyl disulfide moiety for coupling to molecules with a free ...
Reinhard Bredehorst +6 more
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A novel, highly water-soluble, heterobifunctional cross-linking reagent, S-(2-thiopyridyl)-L-cysteine hydrazide (TPCH), was synthesized which contains a hydrazide moiety for coupling to aldehyde groups generated in the carbohydrate residues of antibodies by mild periodate oxidation, and a pyridyl disulfide moiety for coupling to molecules with a free ...
Reinhard Bredehorst +6 more
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[9] Synthesis and applications of heterobifunctional photocleavable cross-linking reagents
1998This study designed, synthesized, and characterized a number of new heterobifunctional photocleavable cross-linking reagents that may be used to photomodulate the activity of proteins or to prepare caged fluorescent dyes. Biomolecules or fluorophores caged via a thiol group with the BNBASE reagent can be covalently linked to a second protein, ligand ...
Johannes Ottl, Gerard Marriott
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Polymer, 1996
Intramolecular cross-links were introduced into collagen molecules with six kinds of cross-linking reagents at a dilute concentration of protein where intermolecular cross-links rarely occurred. Cross-linked collagen molecules regenerated the triple-helical structure from the heat-denatured conformation.
Junko Nakagawa +3 more
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Intramolecular cross-links were introduced into collagen molecules with six kinds of cross-linking reagents at a dilute concentration of protein where intermolecular cross-links rarely occurred. Cross-linked collagen molecules regenerated the triple-helical structure from the heat-denatured conformation.
Junko Nakagawa +3 more
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Semi-Reversible Cross-Linking. Synthesis and Application of a Novel Heterobifunctional Reagent
Hoppe-Seyler´s Zeitschrift für physiologische Chemie, 1981The synthesis of a novel heterobifunctional cross-linking reagent, 3-(4-azido-2-nitrobenzoylseleno)propionic acid is described. The selenol ester function serves as a selective and reversible thiol reagent. Cross-linking is carried out photochemically via nitrene generation.
Klaus Friebel +3 more
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