Results 161 to 170 of about 1,176 (175)
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Food Hydrocolloids, 2016
Abstract Encapsulation of bioactive compounds is an emerging technique to provide protection against food processing and digestion as well as to increase their bioavailability. In this study, cruciferin, a major canola protein, was used to prepare calcium-induced nanoparticles by a cold gelation method. The particles were spherical in shape with ∼200
Ali Akbari, Jianping Wu
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Abstract Encapsulation of bioactive compounds is an emerging technique to provide protection against food processing and digestion as well as to increase their bioavailability. In this study, cruciferin, a major canola protein, was used to prepare calcium-induced nanoparticles by a cold gelation method. The particles were spherical in shape with ∼200
Ali Akbari, Jianping Wu
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THE EFFECT OF pH ON RAPESEED GLOBULIN (CRUCIFERIN) BINDING TO ANILINONAPHTHALENE-8-SULFONATE
Journal of Food Biochemistry, 1995The fluorescence emission intensity from rapeseed globulin (cruciferin) increased in the presence of anilinonaphthalene-8-sulfonate (ANS) at pH 2.0 but not at pH 10. Fluorescence titration studies showed that at pH 7 cruciferin binds 22 (±0.6) moles of ANS per mole of protein with an average dissociation constant (K d ) of 1.9 (±0.1) x 10 -5 M.
RICHARD K. OWUSU APENTEN +1 more
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Food Research International, 2020
The 11S globulin cruciferin is the major storage protein in Brassicaceae/Cruciferae seeds and exists as a hexamer in its natural configuration. Arabidopsis thaliana cruciferin is composed of CRUA, CRUB and CRUC subunits. Wild type (WT) cruciferin and cruciferins composed only of identical CRUA, CRUB and CRUC subunits were examined for their ability to ...
Thushan S, Withana-Gamage +5 more
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The 11S globulin cruciferin is the major storage protein in Brassicaceae/Cruciferae seeds and exists as a hexamer in its natural configuration. Arabidopsis thaliana cruciferin is composed of CRUA, CRUB and CRUC subunits. Wild type (WT) cruciferin and cruciferins composed only of identical CRUA, CRUB and CRUC subunits were examined for their ability to ...
Thushan S, Withana-Gamage +5 more
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Plant Molecular Biology, 2015
Trafficking of seed storage proteins to protein storage vacuoles is mediated by carboxy terminal and internal sorting determinants (ISDs). Protein modelling was used to identify candidate ISDs residing near surface-exposed regions in Arabidopsis thaliana cruciferin A (AtCruA). These were verified by AtCruA fusion to yellow fluorescent protein (YFP) and
Dwayne D, Hegedus +5 more
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Trafficking of seed storage proteins to protein storage vacuoles is mediated by carboxy terminal and internal sorting determinants (ISDs). Protein modelling was used to identify candidate ISDs residing near surface-exposed regions in Arabidopsis thaliana cruciferin A (AtCruA). These were verified by AtCruA fusion to yellow fluorescent protein (YFP) and
Dwayne D, Hegedus +5 more
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Physiologia Plantarum, 1990
Cruciferin (12S globulin) is a large, neutral, oligomeric protein synthesized in rapeseed (Brassica napus) during the seed development. It is composed of six subunit pairs. Each pair consists of one heavy α chain (30 kDa) and one light β chain (20 kDa). Four different subunit pairs exist.
Joakim Rödin, Lars Rask
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Cruciferin (12S globulin) is a large, neutral, oligomeric protein synthesized in rapeseed (Brassica napus) during the seed development. It is composed of six subunit pairs. Each pair consists of one heavy α chain (30 kDa) and one light β chain (20 kDa). Four different subunit pairs exist.
Joakim Rödin, Lars Rask
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pH and NaCl dependent complexation between rapeseed cruciferin and napin
Food HydrocolloidsThe interaction between the main protein components of rapeseed, also called canola, was investigated in water as a function of the pH and in the presence of NaCl. Firstly, the solubility of purified cruciferin and napin was determined and the soluble fraction was characterized using light scattering techniques.
Colleen P.K. Mudau +4 more
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Food Biophysics, 2014
This study examined the solubility, heat-induced gel formation ability, and pepsin susceptibility of cruciferin with mixed (wild type, WT) or identical subunits (CRUA, CRUB, or CRUC). All cruciferin species exhibited minimal solubility at pH 4.0 and slight solubility between pH 7.5 and 8, which was not influenced by the presence of NaCl.
Thushan S. Withana-Gamage +3 more
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This study examined the solubility, heat-induced gel formation ability, and pepsin susceptibility of cruciferin with mixed (wild type, WT) or identical subunits (CRUA, CRUB, or CRUC). All cruciferin species exhibited minimal solubility at pH 4.0 and slight solubility between pH 7.5 and 8, which was not influenced by the presence of NaCl.
Thushan S. Withana-Gamage +3 more
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Plant Molecular Biology, 1993
The major storage protein in seeds of Brassica napus, the 12S globulin cruciferin, is composed of three different groups of subunits; cru1, cru2/3 and cru4. By using gene family-specific probes, we have investigated the accumulation, rate of synthesis and spatial distribution of transcripts corresponding to the different groups of cruciferin subunits ...
S, Sjödahl +5 more
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The major storage protein in seeds of Brassica napus, the 12S globulin cruciferin, is composed of three different groups of subunits; cru1, cru2/3 and cru4. By using gene family-specific probes, we have investigated the accumulation, rate of synthesis and spatial distribution of transcripts corresponding to the different groups of cruciferin subunits ...
S, Sjödahl +5 more
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A 13-week sub-chronic dietary toxicity study of a cruciferin-rich canola protein isolate in rats
Food and Chemical Toxicology, 2009The objective was to evaluate the safety of a cruciferin-rich canola protein isolate (Puratein) when fed as a protein source at various dietary levels to rats for 13-weeks. The study included four groups (20 animals/sex/group) of young Sprague Dawley rats. They were fed ad libitum with an AIN-93 G based protein-free diet added respectively with 5%, 10%
Luis A, Mejia +7 more
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Plant Cell Reports, 1994
Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cruciferin gene promoter have been sequenced and analyzed. Promoter fragments with 5' deletions from -2500 to -v202 were fused with the ß-glucuronidase reporter gene and used for Nicotiana tabacum transformation.
P, Bilodeau +2 more
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Cruciferin is the major seed storage protein in Brassica napus. As much as 1.9 kbp of the BnC1 cruciferin gene promoter have been sequenced and analyzed. Promoter fragments with 5' deletions from -2500 to -v202 were fused with the ß-glucuronidase reporter gene and used for Nicotiana tabacum transformation.
P, Bilodeau +2 more
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