Results 191 to 200 of about 6,820 (210)

Differential heliothine susceptibility to Cry1Ac associated with gut proteolytic activity

Pesticide Biochemistry and Physiology, 2019
The Cry1Ac protein is the most active insecticidal toxin from the bacterium Bacillus thuringiensis (Bt) to members of the heliothinae subfamily in Lepidoptera, which includes some of the most devastating pests of corn and cotton worldwide. However, there are wide discrepancies in susceptibility among members of this subfamily in the US.
Heba M. Abdelgaffar, Cris Oppert, Xiaocun Sun, Jessica Monserrate, Juan Luis Jurat-Fuentes   +4 more
openaire   +2 more sources

Functional display of Bacillus thuringiensis Cry1Ac toxin on T7 phage

Journal of Invertebrate Pathology, 2006
The Cry1Ac toxin from Bacillus thuringiensis was displayed on the surface of T7 phage. The cry1Ac gene was fused to the C-terminal end of T7-10B capsid protein and displayed on the surface of T7 phage as revealed by Western blot analysis of the purified phage particles. The T7-Cry1Ac phages retained toxicity against Manduca sexta larvae.
Sabino, Pacheco, Isabel, Gómez, Ryoichi, Sato, Alejandra, Bravo, Mario, Soberón   +4 more
openaire   +2 more sources

An immuno-PCR method for detecting Bacillus thuringiensis Cry1Ac toxin

Journal of Immunological Methods, 2006
The Cry1Ac toxin is an insecticidal protein produced by Bacillus thuringiensis var. kurstaki. Recently, the gene encoding the toxin was genetically transformed into crop plants. A specific and sensitive method for detecting the Cry1Ac toxin would facilitate monitoring for this protein in crop and non-crop plants and also in foods.
Rebekah C, Allen, Snezna, Rogelj, Susan E, Cordova, Thomas L, Kieft   +3 more
openaire   +2 more sources

Bt cotton expressing Cry1Ac/Cry2Ab or Cry1Ac/epsps does not harm the predator Propylaea japonica through its prey Aphis gossypii

Agriculture, Ecosystems & Environment, 2013
Abstract To assess the ecological effects of Bt -cotton cultivars (ZMSJ and ZMKCKC, expressing Cry1Ac/Cry2Ab and Cry1Ac/EPSPS, respectively), laboratory experiments were performed to evaluate the transmission of Cry1Ac protein in the food chain and the effects of Bt toxin on the ladybeetle predator Propylaea japonica through its herbivorous prey
Yao Zhao, Yan Ma, Lin Niu, Weihua Ma, Amani Mannakkara, Lizhen Chen, Chaoliang Lei   +6 more
openaire   +1 more source

Transgenic Bt cotton expressing Cry1Ac/Cry2Ab or Cry1Ac/EPSPS does not affect the plant bug Adelphocoris suturalis or the pollinating beetle Haptoncus luteolus

Environmental Pollution, 2018
The widespread cultivation of transgenic Bt cotton makes assessing the potential effects of this recombinant crop on non-target organisms a priority. However, the effect of Bt cotton on many insects is currently virtually unknown. The plant bug Adelphocoris suturalis is now a major pest of cotton in southern China and the beetle Haptoncus luteolus is ...
Lin, Niu, Zhenya, Tian, Hui, Liu, Hao, Zhou, Weihua, Ma, Chaoliang, Lei, Lizhen, Chen   +6 more
openaire   +2 more sources

Death-Associated LIM-Only Protein Reduces Cry1Ac Toxicity by Sequestration of Cry1Ac Protoxin and Activated Toxin in Helicoverpa armigera

Journal of Agricultural and Food Chemistry
The extensive use of Bacillus thuringiensis (Bt) in pest management has driven the evolution of pest resistance to Bt toxins, particularly Cry1Ac. Effective management of Bt resistance necessitates a good understanding of which pest proteins interact with Bt toxins.
Yunpeng Duan, Xue Yao, Pin Li, Yuge Zhao, Bo Zhang, Shiheng An, Jizhen Wei, Xianchun Li   +7 more
openaire   +2 more sources

Surface Plasmon Resonance Detection of Transgenic Cry1Ac Cotton (Gossypium spp.)

Journal of Agricultural and Food Chemistry, 2013
The detection and identification of genetically modified (GM) plants are challenging issues that have arisen from the potential negative impacts of extensive cultivation of transgenic plants. The screening process is a long-term focus and needs specific detection strategies.
Zhuoya, Zhao, Yanshan, Chen, Wenzhong, Xu, Mi, Ma   +3 more
openaire   +2 more sources

Antisera-mediated in vivo reduction of Cry1Ac toxicity in Helicoverpa armigera

Journal of Insect Physiology, 2010
A functional assessment of Bacillus thuringiensis (Bt) toxin receptors in the midgut of lepidopteran insects will facilitate understanding of the toxin mode of action and provide effective strategies to counter the development of resistance. In this study, we produced anti-aminopeptidase (APN) and anti-cadherin sera with purified Cry1Ac toxin-binding ...
Chenxi, Liu, Yulin, Gao, Changming, Ning, Kongming, Wu, Brenda, Oppert, Yuyuan, Guo   +5 more
openaire   +2 more sources

EXPRESSION OF CRY1AC AND GUS IN CABBAGE AND CAULIFLOWER

Acta Horticulturae, 2003
Studies on the expression of a GUS reporter gene and a cry1Ac gene under the control of the proprietary tCUP gene expression system have been carried out in vegetable crucifers. The tCUP gene expression system is comprised of a cryptic gene activation sequence isolated from tobacco by T-DNA tagging and has been optimized for expression in cauliflower ...
H. Wang, E. Tsang, J. McNeil, C. Hannam, D.C.W. Brown, B. Miki   +5 more
openaire   +1 more source

Nitrogen deficit decreases seed Cry1Ac endotoxin expression in Bt transgenic cotton

Plant Physiology and Biochemistry, 2019
The spatial and temporal expression of insecticidal gene Cry1Ac in transgenic Bacillus thuringiensis (Bt) cotton varies with plant organs, plant age, as well as environmental conditions. The research was undertaken to study the impact of nitrogen deficit on Cry1Ac endotoxin concentration in seed in Yangtze River valley region in China in 2015 and 2016.
Yuan, Chen, Yabing, Li, Mingyuan, Zhou, Zezhou, Cai, Leila I M, Tambel, Xiang, Zhang, Yuan, Chen, Dehua, Chen   +7 more
openaire   +2 more sources