Design of Ctenophore Ca2+-Regulated Photoprotein Berovin Capable of Being Converted into Active Protein Under Physiological Conditions: Computational and Experimental Approaches [PDF]
Here, we describe (1) the AlphaFold-based structural modeling approach to identify amino acids of the photoprotein berovin that are crucial for coelenterazine binding, and (2) the production and characterization of berovin mutants with substitutions of ...
Ludmila P. Burakova +4 more
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The key residue responsible for the red shift of bioluminescence spectra of light-sensitive Ca2+-regulated photoproteins of ctenophores [PDF]
Isoforms of some ctenophore photoproteins show different maxima of bioluminescence spectra— the ones of mnemiopsin and bolinopsin have λmax at 490 and 500 nm, while λmax for velamin isoforms are at 500 and 508 nm.
Ludmila P. Burakova, Eugene S. Vysotski
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Neural connectome of the ctenophore statocyst [PDF]
Ctenophores possess a unique gravity receptor (statocyst) in their aboral organ formed by four clusters of ciliated balancer cells that collectively support a statolith.
Kei Jokura +4 more
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Neuromuscular organization of the benthic ctenophore, Vallicula multiformis [PDF]
Ctenophora is the earliest metazoan taxon with neurons and muscles. Recent studies have described genetic, physiological, and cellular characteristics of the neural and muscular systems of this phylogenically important lineage.
Kurato Mohri, Hiroshi Watanabe
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Mint/X11 PDZ domains from non-bilaterian animals recognize and bind CaV2 calcium channel C-termini in vitro [PDF]
PDZ domain mediated interactions with voltage-gated calcium (CaV) channel C-termini play important roles in localizing membrane Ca2+ signaling. The first such interaction was described between the scaffolding protein Mint-1 and CaV2.2 in mammals. In this
Alicia N. Harracksingh +13 more
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Bacterial degradation of ctenophore Mnemiopsis leidyi organic matter [PDF]
Blooms of gelatinous zooplankton, an important source of protein-rich biomass in coastal waters, often collapse rapidly, releasing large amounts of labile detrital organic matter (OM) into the surrounding water.
Eduard Fadeev +6 more
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Conserved novel ORFs in the mitochondrial genome of the ctenophore Beroe forskalii [PDF]
To date, five ctenophore species’ mitochondrial genomes have been sequenced, and each contains open reading frames (ORFs) that if translated have no identifiable orthologs. ORFs with no identifiable orthologs are called unidentified reading frames (URFs).
Darrin T. Schultz +5 more
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Ctenophores are quick responders to coastal environmental changes and play a crucial role in marine food web dynamics. We report the environmental drivers of a ctenophore swarm (Pleurobrachia spp.) and associated ecological changes in estuarine and ...
Alfisa Siddique +4 more
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Flow cytometry methods for targeted isolation of ctenophore cells
Cell suspension fluidics, such as flow cytometry (FCS) and fluorescence-activated cell sorting (FACS), facilitates the identification and precise separation of individual cells based on phenotype.
Abigail C. Dieter +9 more
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The Flexiscope: a low cost, flexible, convertible and modular microscope with automated scanning and micromanipulation [PDF]
With technologies rapidly evolving, many research institutions are now opting to invest in costly, high-quality, specialized microscopes which are shared by many researchers.
Amy Courtney +4 more
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