Results 81 to 90 of about 2,851,421 (303)
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Editorial: Grief Disorders: Clinical, Cultural, and Epidemiological Aspects
Geert E. Smid +3 more
doaj +1 more source
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li +8 more
wiley +1 more source
Cultural Aspects of Trade Dispute Resolution in China [PDF]
The increased level of economic interaction in the Pacific Rim region has brought with it a corresponding increase in the number of potential trade disputes. This paper describes a conceptual and methodological framework for examining cultural aspects of
Potter, Pitman
core
HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley +1 more source
TENARERE: A CULTURAL TRADITION SONG AND DANCE FOR STRENGTHENING BROTHERHOOD UNITY OF ADONARA COMMUNITY MEMBERS IN EAST NUSA TENGGARA [PDF]
Tenarere as a cultural art tradition of the ethnic group in Adonara area (East Nusa Tenggara) belongs to a ritual dance. The performance of this art form is used as an annual celebration for recalling the historical journey of their great achestors ...
I Nyoman, Suteja
core
British and Pakistani children's understanding of death: Cultural and developmental influences [PDF]
This study explored British and Pakistani 4- to 7-year-olds’ (N = 188 understanding of death. The aim was to examine possible influences on the acquisition of the subcomponents of the death concept by investigating how they are understood by children of ...
Ashraf, Aisha +3 more
core +1 more source
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti +3 more
wiley +1 more source

