Results 81 to 90 of about 2,851,421 (303)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Editorial: Grief Disorders: Clinical, Cultural, and Epidemiological Aspects

open access: yesFrontiers in Psychiatry, 2021
Geert E. Smid   +3 more
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

open access: yesFEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li   +8 more
wiley   +1 more source

Editorial: How to better understand and treat children and adolescents suffering from eating disorders

open access: yesFrontiers in Psychiatry, 2023
Fiona McNicholas   +6 more
doaj   +1 more source

Cultural Aspects of Trade Dispute Resolution in China [PDF]

open access: yes
The increased level of economic interaction in the Pacific Rim region has brought with it a corresponding increase in the number of potential trade disputes. This paper describes a conceptual and methodological framework for examining cultural aspects of
Potter, Pitman
core  

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

open access: yesFEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

TENARERE: A CULTURAL TRADITION SONG AND DANCE FOR STRENGTHENING BROTHERHOOD UNITY OF ADONARA COMMUNITY MEMBERS IN EAST NUSA TENGGARA [PDF]

open access: yes, 2008
Tenarere as a cultural art tradition of the ethnic group in Adonara area (East Nusa Tenggara) belongs to a ritual dance. The performance of this art form is used as an annual celebration for recalling the historical journey of their great achestors ...
I Nyoman, Suteja
core  

British and Pakistani children's understanding of death: Cultural and developmental influences [PDF]

open access: yes, 2014
This study explored British and Pakistani 4- to 7-year-olds’ (N = 188 understanding of death. The aim was to examine possible influences on the acquisition of the subcomponents of the death concept by investigating how they are understood by children of ...
Ashraf, Aisha   +3 more
core   +1 more source

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

open access: yesFEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti   +3 more
wiley   +1 more source

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