Results 81 to 90 of about 5,461,349 (295)
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli +3 more
wiley +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Understanding India - South Africa tourism: The Impact of Food Tourism on the Socio-Cultural and Economic Fabrics in Durban. [PDF]
India-Africa relations is distinct and owes its origins to a common past that the countries share. In this paper the researchers attempt to trace India’s engagement in South Africa and its socio-cultural and economic impact in the coastal city of Durban.
Joram Ndlovu, Vivian B. Ojong
doaj
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Children’s Literature in Translation: Towards a Participatory Approach
In the Netherlands and Flanders, more or less a fifth of all children’s books are translations. The decision of what gets translated and funded is, for the most part, informed by adults’ decisions.
Vanessa Joosen
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Índices de impacto cultural: antecedentes, metodología y resultados
El resultado que se registra en el presente documento es un hallazgo metodológico para la definición de una batería de indicadores de efecto e impacto cultural y para la construcción de índices de impacto cultural.
Banco de la República (Bogotá). Subgerencia Cultural
core
Beyond Borders: Mindol Qutuɣtu and His Early Approach to Combined Medical Practice
The spread of Tibetan Buddhism in Mongolia brought with it a wealth of Buddhist knowledge. Over time, Mongolian scholars and practitioners engaged with this knowledge and produced numerous works encompassing Buddhist learning, particularly in medicine. A
Tsetsenbaatar Gunsennyam +3 more
doaj +1 more source
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia +9 more
wiley +1 more source

