HSP70 governs permeability and mechanotransduction in primary human endothelial cells
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez +5 more
wiley +1 more source
Comparison of culture media supplements identifies serum components in self-reported serum-free preparations. [PDF]
Delabie W +5 more
europepmc +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Development of selective culture media for efficient isolation of <i>Avibacterium paragallinarum</i> from chickens. [PDF]
Srednik ME +7 more
europepmc +1 more source
CONIDIUM PRODUCTION OF VENTURIA INAEQUALIS IN SYNTHETIC CULTURE MEDIA [PDF]
R. G. Ross
openalex +1 more source
Homologous expression and purification of human HAX‐1 for structural studies
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Rescue in vitro maturation of germinal vesicle oocytes after ovarian stimulation: the importance of the culture media. [PDF]
Soler N +8 more
europepmc +1 more source
Interim Report by the Committee on Youth, Culture, Education, the Media and Sport on the movement of objects of cultural interest in the context of the single market. Session Documents 1990, Document A3-0324/90, 28 November 1990 [PDF]
Marc Galle
openalex

