Culture of unpollinated ovules in vitro

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Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo

Plant Cell, Tissue and Organ Culture, 1998
Ovaries from squash plants (cv. Eskandarani) were picked one day before anthesis, and exposed to cold temperature (4 °C) for 0, 2, 4 and 8 days. The ovules were cultured on MS medium with 30 g l−1sucrose, 8 g l−1agar and supplemented with four concentrations of 2,4-D, i.e., 0.1, 1.0, 5 and 10 mg l−1. Then the dishes were incubated at 25 ± 1 °C under 16-
E.I. Metwally +4 more
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Gynogenesis in Beta vulgaris L.: From in vitro culture of unpollinated ovules to the production of doubled haploid plants in soil

Plant Cell Reports, 1985
Our study concerns the different stages involved in the production of doubled haploid beet plants by in vitro gynogenesis. A histological study shows that the embryos obtained could come from the oosphere or the antipodals. Gynogenesis using male fertile plants gives nearly two haploid plants for 1000 ovules cultured: in this case, the problem is to ...
D, Bossoutrot, D, Hosemans
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Induction of autonomous endosperm development in ovules of unpollinated pistils of Arabidopsis thaliana var. Landsberg cultured in vitro [PDF]

Acta Biologica Cracoviensia Series Botanica, 2007
The paper reports induction of autonomous endosperm (AE) development in ovules of unpollinated pistils of Arabidopsis thaliana var. Landsberg cultured in vitro. The basic nutritive medium was hormone-free Murashige and Skoog (MS) medium with 6% sucrose added.
Małgorzata Kapusta, Joanna Rojek, Jerzy Bohdanowicz +2 more
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Production of doubled haploid plants ofCucurbitaceaefamily crops through unpollinated ovule culture in vitro