FEBS Open Bio, EarlyView.The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida +6 more
wiley +1 more source
Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes
FEBS Open Bio, EarlyView.We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde +3 more
wiley +1 more source
Optimization of 5-axis milling processes using process models [PDF]
, 2006 Productivity and part quality are extremely important for all machining operations, but particularly for 5-axis milling where the machine tool cost is relatively higher, and most parts have complex geometries and high quality requirements with tight ...
Budak, Erhan +4 more
core
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Experimental Investigation and Statistical Evaluation of Optimized Cutting Process Parameters and Cutting Conditions to Minimize Cutting Forces and Shape Deviations in Al6026-T9. [PDF]
Materials (Basel), 2020 Abas M +9 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Analysis and modeling of green wood milling: Chip production by slabber [PDF]
, 2014 During the primary transformation of wood, logs are faced with slabber heads. Chips produced are raw materials for pulp paper and particleboard industries. Efficiency of these industries is partly due to particle size distribution.
COLLET, Robert +4 more
core +1 more source
Investigation of cutting conditions on tool life in shoulder milling of Ti6Al4V using PVD coated micro-grain carbide insert based on design of experiments. [PDF]
Heliyon, 2020 Tatar K, Sjöberg S, Andersson N.
europepmc +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
On the Chip Shaping and Surface Topography When Finish Cutting 17-4 PH Precipitation-Hardening Stainless Steel under Near-Dry Cutting Conditions. [PDF]
Materials (Basel), 2020 Leksycki K +3 more
europepmc +1 more source