Results 201 to 210 of about 1,424,201 (360)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Dry Machining of Inconel 713LC: Surface Integrity and Force Response to Cutting Conditions. [PDF]

Materials (Basel)
Slaný M, Mádl J, Pitrmuc Z, Sommer J, Stránský O, Beránek L.   +5 more
europepmc   +1 more source

Modelling of the Relationship of Adiabatic Shear and Cutting Conditions for Higher Cutting Speed Ranges [PDF]

, 2017
Guohe Li, Mohan Li, Yujun Cai -Houjun, Qi -Meng Liu, J Barry, G Byrne, G Ye, S Xue, W Ma, M Jiang, Z Ling, X Tong, L Dai, A Vyas, M Shaw, R Recht, R Komandui, T Schroeder, J Hazra, B Von Turkovich, D Flom, M Davies, T Burns, C Evans, A Molinari, C Musquar, G Sutter, W Ma, X Li, L Dai, Z Ling, J Sheikh-Ahmad, V Quarless, J Bailey, S Sun, M Brandt, M Dargusch, J Huang, K Kalaitzidou, J Sutherland, E Aifantis, C Duan, L Zhang, A Molinari, X Soldani, M Migulez, M Migulez, X Soldani, A Molinari, L Gu, M Wang, C Duan, L Gu, G Kang, H Chen, M Wang, G Ye, S Xue, M Jiang, G Ye, Y Chen, S Xue, Dai, G Ye, M Jiang, S Xue, W Ma, L Dai, C Wang, Y Xie, L Zheng, Z Qin, D Tang, Y Song, H Wu, S To, W Jomaa, O Mechri, J Lvesque, V Songmene, P Bocher, A Gakwaya, B Wang, Z Liu, B Wang, Z Liu, B Wang, Z Liu, G Li, M Wang, C Duan, J Th, R Scrutton, A Tay, M Stevenson, G De Vahl Davis, P Oxley, G Li, M Wang, C Duan   +99 more
openalex   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Effect of Cutting Conditions on the Size of Dust Particles Generated During Drilling of Carbon Fiber Reinforced Composite Systems. [PDF]

Polymers (Basel)
Knápek T, Dvořáčková Š, Kroisová D, Váňa M.   +3 more
europepmc   +1 more source

Study on milling of super elasto-plastic β type titanium alloy (Verification of processing characteristics and selection of optimum cutting conditions)

, 2017
Hideharu KATO, Kohei Ito, Akihiro Kitamura, Noriaki Ikenaga, K. Kubota   +4 more
openalex   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Characterizing the tool wear morphologies and life in milling A520-10%SiC under various lubrication and cutting conditions. [PDF]

Sci Rep
Saberi M, Niknam SA, Hashemi R.
europepmc   +1 more source

Growth, Yield and Economics of Acorus calamus L. as Influenced by Different Node Cuttings under Mid-Hill Conditions of Himachal Pradesh

, 2020
Karma Gyalpo Bhutia, Meenu Sood, Vinod Kumar, Pempa Lamu Bhutia, Pradeepen Rai   +4 more
openalex   +1 more source

Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment

Aging and Cancer, EarlyView.
Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey, Murilo Ramos Rocha, Cheyenne Palm, Vania Wang, Denys Balandin, Ethan Black, Kevin Y. Zhang, Fan Huang, Daniel J. Zabransky, Yash Chhabra, Mareike Peters, Gabriel Ghiaur, Ashani Weeraratna   +12 more
wiley   +1 more source