Multiscale Simulation and Machine Learning Optimization of Cuttings Transport in Riserless Pipelines Under Bubble Coalescence-Breakup Driven Flow. [PDF]
Xiang H, Zhang G, Zhang S, Zhang Y.
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Laboratory Protocols for Candidatus Liberibacter asiaticus Transmission Biology and Management Research. [PDF]
Galdeano DM +5 more
europepmc +1 more source
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Commodity risk assessment of <i>Salix</i> unrooted cuttings from the UK. [PDF]
EFSA Panel on Plant Health (PLH) +29 more
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Rooting Ability of Eucalyptus dunnii Maiden Mini-Cuttings Is Conditioned by Stock Plant Nighttime Temperature. [PDF]
Nión M +8 more
europepmc +1 more source
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
ABT Promotes Adventitious Root Formation in Mulberry Cuttings by Coordinating Hormonal Homeostasis and Defense Priming. [PDF]
Qin Z +12 more
europepmc +1 more source

