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Crystallization of Cyclic Nucleotide Phosphodiesterases
2005Selective inhibitors of cyclic nucleotide phosphodiesterases (PDEs) have been widely studied as therapeutic agents for the treatment of various human diseases. Three-dimensional structures are essential for the design of highly selective inhibitors, but their availability is limited by the speed of crystallization.
Robert X. Xu, Qing Huai, Hengming Ke
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Cyclic nucleotide phosphodiesterases in uterine development
Molecular and Cellular Endocrinology, 1975Activities of cyclic nucleotide phosphodiesterase were studied in rat uterus as a function of age, DNA and protein content. Linear kinetics were observed for uterine homogenate cyclic GMP (cGMP) phosphodiesterase activity, but anomalous double-reciprocal plots, suggestive of multiple enzyme forms, were observed for cyclic AMP (cAMP) hydrolysis, cAMP ...
George M. Stancel +2 more
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THE DISTRIBUTION OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE IN THE HYPOTHALAMUS
Journal of Neurochemistry, 1978Abstract— Quantitative measurements of capacities of microgram‐sized samples of tissue to hydrolyze adenosine 3′,5′‐phosphate (cyclic AMP) and guanosine 3′,5′‐phosphate (cyclic GMP) have been made in 32 discrete regions of the rabbit hypothalamus. The capacities to hydrolyze the two cyclic nucleotides were found to be distributed over a 3‐fold range ...
Allyn C. Howlet, Bruce McL. Breckenridge
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Cyclic nucleotide phosphodiesterase in pea seedlings
Biochimica et Biophysica Acta (BBA) - Enzymology, 1972Abstract An enzyme from pea seedlings that hydrolyzes both 2′,3′-cyclic nucleoside monophosphate and 3′,5′-cyclic nucleoside monophosphate has been partially purified from pea seedlings. It has a molecular weight of 350 000 and has optimal activity at pH 5.4–6.0. It is insensitive to methylxanthines and imidazole. It catalyzes the formation of 3′-AMP
Joseph E. Varner, Paul Po-Chao Lin
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Cyclic Nucleotide Phosphodiesterase Assay Technology
Current Protocols in Pharmacology, 2010AbstractBecause of their critical role in modulating cellular cyclic nucleotide levels, phosphodiesterases (PDEs) are involved in many disease‐related signaling pathways. The PDE family is large and diverse, with members having different tissue distribution, sub‐cellular localizations, and substrate specificities.
Christopher Wells +2 more
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Cyclic nucleotide phosphodiesterases in the human lung
Lung, 1994Although theophylline has been used in the treatment of lung diseases, particularly bronchial asthma, since the nineteenth century, the mechanisms underlying its effectiveness remained poorly understood until quite recently. The identification of cyclic nucleotide phosphodiesterase (PDE)--the enzyme responsible for breaking down cyclic AMP and cyclic ...
Klaus F. Rabe, G. Dent, Helgo Magnussen
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Cyclic nucleotide phosphodiesterase in silkworm
Biochimica et Biophysica Acta (BBA) - Enzymology, 1975The existence of cyclic GMP phosphodiesterase (EC 3.1.4.-) was demonstrated in silkworm larva by gel filtration of the homogenate. The cyclic GMP phosphodiesterase was separated from cyclic AMP phosphodiesterases by column chromatography on hydroxyapatite and Sephadex G-200. The enzyme has a molecular weight of approx.
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Cyclic nucleotide phosphodiesterase of Chlamydomonas reinhardtii
Biochimica et Biophysica Acta (BBA) - Enzymology, 1974Abstract Cyclic nucleotide phosphodiesterase (EC 3.1.4.17) activity was characterized in crude and (NH4)2SO4-fractionated extracts of the green alga Chlamydomonas reinhardtii. With cyclic AMP as substrate, the formation of 5′-AMP was optimal at pH 8.5 and required the presence of a sulfhydryl reagent and a divalent cation, Mg2+ and Mn2+ being the ...
Ulrich Fischer, Nikolaus Amrhein
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Cyclic nucleotide phosphodiesterase from Tetrahymena
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1973Abstract The existence of multiple molecular forms of a cyclic nucleotide phosphodiesterase in Tetrahymena pyriformis is shown.
S.C. Chou, S. Ramanathan
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Cyclic nucleotide phosphodiesterases in plants
Zeitschrift für Pflanzenphysiologie, 1974Summary Hydrolysis of cyclic AMP was catalyzed by extracts from various algae and plants of different levels of organization ( Bryophyta, Pteridophyta, Spermatophyta ). The 3′-ester linkage of cyclic AMP was cleaved by the enzymes of algal origin giving rise to 5′-AMP as the product of hydrolysis, which was optimal in the alkaline pH range ...
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