Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
A predictive descriptor for the d-band center in intermetallic alloys accelerates the design of robust molecular switches. [PDF]
Yang S +5 more
europepmc +1 more source
4Gbaud PS-16QAM D-Band Fiber-Wireless Transmission over 4.6 km by Using Balance Complex-Valued NN Equalizer with Random Oversampling. [PDF]
Xie T, Yu J.
europepmc +1 more source
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Tailoring Intermediate Adsorption Through d-band Mismatch Strategy of Heterojunction to Achieve Industrial Overall Water Splitting. [PDF]
Liu J +10 more
europepmc +1 more source
Trimetallic Sulfide Hollow Superstructures with Engineered d-Band Center for Oxygen Reduction to Hydrogen Peroxide in Alkaline Solution. [PDF]
Zhang C +9 more
europepmc +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Bidirectional catalysts with dual-atom dynamic d-band centre modulation and support self-reconstruction for de/hydrogenation in MgH<sub>2</sub>/Mg. [PDF]
Jin J +12 more
europepmc +1 more source
In situ modification of the d-band in the core-shell structure for efficient hydrogen storage via electrocatalytic N2 fixation. [PDF]
Yang X, Wan J, Zhang H, Wang Y.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source

