Results 161 to 170 of about 16,379,393 (306)
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Outperforming completely randomized designs. [PDF]
Bi-randomization designs have become increasingly popular in industry because some of the factors under investigation are often hard-to-change. It is well-known that the resulting compound symmetric error structure not only affects estimation and ...
Goos, Peter, Vandebroek, Martina
core
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Optimal designs for 3D shape analysis with spherical harmonic descriptors [PDF]
We determine optimal designs for some regression models which are frequently used for describing 3D shapes. These models are based on a Fourier expansion of a function defined on the unit sphere in terms of spherical harmonic basis functions.
Dette, Holger +2 more
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
On efficiency of locally <i>D</i>-optimal designs under heteroscedasticity and non-Gaussianity. [PDF]
J Appl StatZhang X, Shen G.
europepmc +1 more source
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Optimal design for goodness-of-fit of the Michaelis-Menten enzyme kinetic function [PDF]
We construct efficient designs for the Michaelis-Menten enzyme kinetic model capable of checking model assumption. An extended model, called EMAX model is also considered for this purpose.
Dette, Holger +2 more
core
D-optimal designs for two-variable logistic regression model with restricted design space. [PDF]
Commun Stat Theory MethodsZhai Y, Wang C, Lin HY, Fang Z.
europepmc +1 more source