Results 161 to 170 of about 231,256 (313)
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
D-OPTIMAL DESIGNS WITH ORDERED CATEGORICAL DATA
, 2017 Cumulative link models have been widely used for ordered categorical responses. Uniform allocation of experimental units is commonly used in practice, but often suffers from a lack of efficiency.
LP Tong (7937435) +2 more
core +1 more source
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Optimal design for goodness-of-fit of the Michaelis-Menten enzyme kinetic function [PDF]
We construct efficient designs for the Michaelis-Menten enzyme kinetic model capable of checking model assumption. An extended model, called EMAX model is also considered for this purpose.
Dette, Holger +2 more
core
Computing Experiment-Constrained D-Optimal Designs
In optimal experimental design, the objective is to select a limited set of experiments that maximizes information about unknown model parameters based on factor levels. This work addresses the generalized D-optimal design problem, allowing for nonlinear relationships in factor levels.
Aditya Pillai +5 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
Model-robust and model-sensitive designs. [PDF]
: The main drawback of the optimal design approach is that it assumes the statistical model is known. In this paper, a new approach to reduce the dependency on the assumed model is proposed.
Goos, Peter +3 more
core
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
FEBS Open Bio, EarlyView.The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Optimal designs for dose-response models with restricted design spaces [PDF]
In dose response studies, the dose range is often restricted due to concerns over drug toxicity and/or efficacy. We present restricted and unrestricted interval locally optimal designs with respect to a very general class of optimality criteria for ...
Zhu, Wei +2 more
core
On efficiency of locally <i>D</i>-optimal designs under heteroscedasticity and non-Gaussianity. [PDF]
J Appl StatZhang X, Shen G.
europepmc +1 more source