Results 71 to 80 of about 100,904 (263)
SD Shafran, JM Conly
openaire +3 more sources
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
6 pages, submitted to ACM MMSys'18 (dataset track)
Anatoliy Zabrovskiy +2 more
openaire +2 more sources
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
The value of open research data (ORD), a key feature of open science, lies in their reuse. However, the mere online availability of ORD does not guarantee their reuse by other researchers.
Franziska Usée +2 more
doaj +1 more source
Introduction. The effect of exercise program on the prevention of upper extremities musculoskeletal disorders has attracted the attention of researchers.
Farzane Fadaei +4 more
doaj
MiR‐513a promotes human erythroid differentiation by modulating c‐Jun
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim +11 more
wiley +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Reactive oxygen species (ROS) are central regulators of plant development and stress responses, with hydrogen peroxide (H2O2) acting as a key signaling molecule whose spatial distribution determines adaptive versus damaging outcomes.
Mario Fenech, Vitor Amorim-Silva
doaj +1 more source

