Results 201 to 210 of about 1,372,066 (309)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

ImageGP 2 for enhanced data visualization and reproducible analysis in biomedical research. [PDF]

open access: yesImeta
Chen T   +15 more
europepmc   +1 more source

Genetic diversity and population structure of the Taigan dog breed

open access: yesFEBS Open Bio, EarlyView.
The Taigan is a rare sighthound from the mountains of Kyrgyzstan. We used DNA markers to study its genetic diversity and compare it with other sighthound breeds. Our findings show that the Taigan shares close genetic ties with the Kazakh Tazy, suggesting a common ancestry shaped by nomadic traditions and regional adaptation.
Kira Bespalova   +10 more
wiley   +1 more source

Effective data visualization strategies in untargeted metabolomics.

open access: yesNat Prod Rep
Mildau K   +12 more
europepmc   +1 more source

Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase

open access: yesFEBS Open Bio, EarlyView.
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni   +4 more
wiley   +1 more source

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