Results 11 to 20 of about 275 (61)

A case for glycerol as an acceptable additive for single‐particle cryoEM samples

open access: yesActa Crystallographica Section D, Volume 78, Issue 1, Page 124-135, January 2022., 2022
It is shown that the inclusion of glycerol in single‐particle cryoEM buffers does not preclude high‐resolution structure determination, as demonstrated by an ∼2.3 Å resolution reconstruction of mouse apoferritin (∼500 kDa) and an ∼3.3 Å resolution reconstruction of rabbit muscle aldolase (∼160 kDa) in the presence of 20%(v/v) glycerol.Buffer ...
Benjamin Basanta   +3 more
wiley   +1 more source

Macromolecular structure determination using X‐rays, neutrons and electrons: recent developments in Phenix

open access: yesActa Crystallographica Section D, Volume 75, Issue 10, Page 861-877, October 2019., 2019
Recent developments in the Phenix software package are described in the context of macromolecular structure determination using X‐rays, neutrons and electrons.Diffraction (X‐ray, neutron and electron) and electron cryo‐microscopy are powerful methods to determine three‐dimensional macromolecular structures, which are required to understand biological ...
Dorothee Liebschner   +24 more
wiley   +1 more source

Standards for MicroED

open access: yesActa Crystallographica Section C, Volume 81, Issue 7, Page 376-390, July 2025.
With an increasing number of refined MicroED structures available to the community, lessons can be learned of their profiles and quality, as well as of best practices.As the electron diffraction technique MicroED gains momentum and is increasingly embraced by researchers in both academia and industry, we have the opportunity to familiarize ourselves ...
Johan Unge   +3 more
wiley   +1 more source

Bias caused by a popular weighting scheme

open access: yesJournal of Applied Crystallography, Volume 58, Issue 1, Page 283-289, February 2025.
The systematic error of systematically too small formal standard uncertainties of the observed intensities cannot be corrected for by the popular SHELXL‐like weighting scheme. Instead, it disguises the error and induces bias in the equivalent isotropic atomic displacement parameters.It is reported how the popular SHELXL‐like weighting scheme leads to ...
Julian Henn
wiley   +1 more source

Why can't we talk about suicide? [PDF]

open access: yesGlob Ment Health (Camb)
Kohrt BA.
europepmc   +1 more source

Has COVID-19 Affected Cancer Screening Programs? A Systematic Review. [PDF]

open access: yesFront Oncol, 2021
Alkatout I   +6 more
europepmc   +1 more source

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