A case for glycerol as an acceptable additive for single‐particle cryoEM samples
It is shown that the inclusion of glycerol in single‐particle cryoEM buffers does not preclude high‐resolution structure determination, as demonstrated by an ∼2.3 Å resolution reconstruction of mouse apoferritin (∼500 kDa) and an ∼3.3 Å resolution reconstruction of rabbit muscle aldolase (∼160 kDa) in the presence of 20%(v/v) glycerol.Buffer ...
Benjamin Basanta +3 more
wiley +1 more source
Recent developments in the Phenix software package are described in the context of macromolecular structure determination using X‐rays, neutrons and electrons.Diffraction (X‐ray, neutron and electron) and electron cryo‐microscopy are powerful methods to determine three‐dimensional macromolecular structures, which are required to understand biological ...
Dorothee Liebschner +24 more
wiley +1 more source
With an increasing number of refined MicroED structures available to the community, lessons can be learned of their profiles and quality, as well as of best practices.As the electron diffraction technique MicroED gains momentum and is increasingly embraced by researchers in both academia and industry, we have the opportunity to familiarize ourselves ...
Johan Unge +3 more
wiley +1 more source
Bias caused by a popular weighting scheme
The systematic error of systematically too small formal standard uncertainties of the observed intensities cannot be corrected for by the popular SHELXL‐like weighting scheme. Instead, it disguises the error and induces bias in the equivalent isotropic atomic displacement parameters.It is reported how the popular SHELXL‐like weighting scheme leads to ...
Julian Henn
wiley +1 more source
Impact of quality routine health data utilisation on health service delivery outcomes in low-income and middle-income countries: a systematic review protocol. [PDF]
Addo P +6 more
europepmc +1 more source
Self-Assembly of a Therapeutic Peptide Surfactant: A Small-Angle X-ray Scattering Study. [PDF]
Brunzell E +2 more
europepmc +1 more source
Proportion Of vitiligo and associated factors among patients visiting dermatology opd in tibebe ghion specialized hospital and addisalem primary hospital, bahirdar city, Ethiopia,2023. [PDF]
Kibret G +4 more
europepmc +1 more source
Has COVID-19 Affected Cancer Screening Programs? A Systematic Review. [PDF]
Alkatout I +6 more
europepmc +1 more source
The Chongqing Adolescent Twin Study: An Integrative Multimodal Brain Imaging and Non-imaging Dataset. [PDF]
Zhu Y +7 more
europepmc +1 more source

