Results 11 to 20 of about 20,554 (165)

Extraction, Structural Characterization and Biological Activities of Polysaccharides Derived From <i>Hericium erinaceus</i>. [PDF]

Food Sci Nutr
This review summarizes the methods used to extract and purify H. erinaceus polysaccharides (HEP), primarily hot water extraction and column chromatography. Structural characterization indicates that the molecular weights of HEP range from 2.1 to 75,000 kDa, with glucose as the dominant monosaccharide and β‐glycosidic bonds, notably β‐glucans with 1 → 3
Sun N, Li C, Liang W, Wu Z, Han X, Xu S.
europepmc   +2 more sources

Historical Insights Into the Purification of Polyphenol Oxidase From Plants. [PDF]

Food Sci Nutr
Overview of purification methods for polyphenol oxidase. Advancements in achieving partial or homogeneous purification. Key biochemical properties relevant to polyphenol oxidase. ABSTRACT Over the past 50 years, different methods have been developed to purify polyphenol oxidase (PPO), a key enzyme involved in the oxidation of phenolic compounds in ...
Vazquez-Lima CG, Quintana-Quirino M, Bravo AL, López-Simeon R, Nájera H.   +4 more
europepmc   +2 more sources

Surface electrical charge of bloodstream trypomastigotes of Trypanosoma cruzi strains

Memorias do Instituto Oswaldo Cruz, 1983
Bloodstream trypomastigotes of some Trypanosoma cruzi strains were processed through DEAE-cellulose columns under standardized conditions. The results obtained suggest mainly that these strains present different surface charges, that there are ...
Maria Auxiliadora de Sousa
doaj   +1 more source

Extraction, isolation and antioxidant activity of the polysaccharides from embryo of the seeds of Nelumbo nucifera Gaertn(莲子心多糖的提取、分离和抗氧化活性研究)

Zhejiang Daxue xuebao. Lixue ban, 2008
干燥的莲子心经机械粉碎、乙醇脱脂、沸水提取、乙醇沉淀及中性酶和Sevag法除蛋白得其粗多糖，经DEAE-Cellulose-32阴离子交换和Sephacryl™ S-200凝胶过滤层析得组份ELPS-Ⅰ和混合物Ⅱ，Ⅱ再经过DEAE-Cellulose-32柱层析得组份ELPS-Ⅱ . GC-MS法测定粗多糖、ELPS-Ⅰ和ELPS-Ⅱ的中性糖组成.凝胶过滤色谱(GPC)法测得ELPS-Ⅰ和ELPS-Ⅱ的重均分子量分别为4.97×104 Da和2.96×106Da.
YUYuan-zhi(俞远志), WUYa-lin(吴亚林), PANYuan-jiang(潘远江)   +2 more
doaj   +1 more source

Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV

Plant Protection Science, 2005
The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV ...
Milada Šindelářová, Luděk Šindelář   +1 more
doaj   +1 more source

Leaf proteins in five varieties of red clover cultivated in Poland

Acta Agrobotanica, 2015
Protein fractions: albumins, globulins, gluteins and prolamins were extracted from the leaves of 5 varieties of red clover. 'Skrzeszowicka' and 'Hruszowska' showed the highest content of total protein, 'Rotra' however – the highest globulin level ...
W. Maciejewska-Potapczyk, L. Konopska, K. Bytniewska, A. Radziwonowska, H. Zawierucha, H. Michniewicz   +5 more
doaj   +1 more source

Isolation and fatty acid composition of the plant sulfolipid and galactolipids*

Journal of Lipid Research, 1964
The plant sulfolipid has been isolated from Chlorella pyrenoidosa cells and from alfalfa leaves by chromatography on Florisil and DEAE cellulose columns.
John S. O'Brien, A.A. Benson
doaj   +1 more source

Enhancing Laccase Production by Trametes hirsuta GMA-01 Using Response Surface Methodology and Orange Waste: A Novel Breakthrough in Sugarcane Bagasse Saccharification and Synthetic Dye Decolorization

Reactions
Trametes hirsuta GMA-01 was cultivated in a culture medium supplemented with orange waste, starch, wheat bran, yeast extract, and salts. The fungus produced several holoenzymes, but the laccase levels were surprisingly high.
Guilherme Guimarães Ortolan, Alex Graça Contato, Guilherme Mauro Aranha, Jose Carlos Santos Salgado, Robson Carlos Alnoch, Maria de Lourdes Teixeira de Moraes Polizeli   +5 more
doaj   +1 more source

Biochemical investigations on endosperm development in Iris pseudoacorus L.

Acta Societatis Botanicorum Poloniae, 2015
In the successive development phases the nitrogen and protein fractions were determined and ion exchanger chromatography of globulins was performed on DEAE cellulose in the endosperm of the chalasal and micropylar poles of Iris pseudoacorus seeds.
L. Konopska
doaj   +1 more source

Production of cold-active pectinases by three novel Cladosporium species isolated from Egypt and application of the most active enzyme

Scientific Reports, 2022
Cladosporium parasphaerospermum, Cladosporium chlamydosporigenum, and Cladosporium compactisporum have all been discovered and characterized as new Cladosporium species.
Ahmad Mohamed Moharram, Abdel-Naser Ahmed Zohri, Abd El-Latif Hesham, Hossam E. F. Abdel-Raheam, Mohamed Al-Ameen Maher, Osama Abdel-Hafeez Al-Bedak   +5 more
doaj   +1 more source