Extraction, Structural Characterization and Biological Activities of Polysaccharides Derived From <i>Hericium erinaceus</i>. [PDF]
This review summarizes the methods used to extract and purify H. erinaceus polysaccharides (HEP), primarily hot water extraction and column chromatography. Structural characterization indicates that the molecular weights of HEP range from 2.1 to 75,000 kDa, with glucose as the dominant monosaccharide and β‐glycosidic bonds, notably β‐glucans with 1 → 3
Sun N, Li C, Liang W, Wu Z, Han X, Xu S.
europepmc +2 more sources
Historical Insights Into the Purification of Polyphenol Oxidase From Plants. [PDF]
Overview of purification methods for polyphenol oxidase. Advancements in achieving partial or homogeneous purification. Key biochemical properties relevant to polyphenol oxidase. ABSTRACT Over the past 50 years, different methods have been developed to purify polyphenol oxidase (PPO), a key enzyme involved in the oxidation of phenolic compounds in ...
Vazquez-Lima CG +4 more
europepmc +2 more sources
Tracking Protein Motions using Serial Femtosecond Crystallography with X-Ray Free-Electron Laser. [PDF]
Abstract Since the birth of biochemistry, researchers have investigated the structure–function relationship of a wide variety of proteins. However, until recently, when X‐ray free‐electron lasers (XFELs) became available, it was not possible to visualize the motion of proteins from moment to moment with excellent temporal and spatial resolution.
Mizohata E +4 more
europepmc +2 more sources
Chitinases from pitcher fluid of Nepenthes distillatoria
Chitinases belong to a diverse family of enzymes among many other hydrolytic enzymes of the pitcher fluid of Nepenthes sp. There are no existing studies on identification of chitinases in Nepenthes distillatoria the only representative of the genus ...
R. W. K. M. Senevirathna +2 more
doaj +1 more source
Surface electrical charge of bloodstream trypomastigotes of Trypanosoma cruzi strains
Bloodstream trypomastigotes of some Trypanosoma cruzi strains were processed through DEAE-cellulose columns under standardized conditions. The results obtained suggest mainly that these strains present different surface charges, that there are ...
Maria Auxiliadora de Sousa
doaj +1 more source
干燥的莲子心经机械粉碎、乙醇脱脂、沸水提取、乙醇沉淀及中性酶和Sevag法除蛋白得其粗多糖,经DEAE-Cellulose-32阴离子交换和Sephacryl™ S-200凝胶过滤层析得组份ELPS-Ⅰ和混合物Ⅱ,Ⅱ再经过DEAE-Cellulose-32柱层析得组份ELPS-Ⅱ . GC-MS法测定粗多糖、ELPS-Ⅰ和ELPS-Ⅱ的中性糖组成.凝胶过滤色谱(GPC)法测得ELPS-Ⅰ和ELPS-Ⅱ的重均分子量分别为4.97×104 Da和2.96×106Da.
YUYuan-zhi(俞远志) +2 more
doaj +1 more source
The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV ...
Milada Šindelářová +1 more
doaj +1 more source
PHYCOERYTHROCYANINS FROM Westiellopsis prolifica AND Nostoc rivulare: CHARACTERIZATION OF THE PHYCOVIOLOBILIN CHROMOPHORE IN BOTH STATES [PDF]
Phycoerythrocyanin or fractions enriched in it have been isolated from the filamentous cyanobacteria, Westiellopsis prolifica ARM 365 and Nostoc rivulare ARM 212.
Braslavsky S. E. +7 more
core +1 more source
The derivation of the formyl-group oxygen of chlorophyll b in higher plants from molecular oxygen. [PDF]
The mechanism of formation of the formyl group of chlorophyll b has long been obscure but, in this paper, the origin of the 7-formyl-group oxygen of chlorophyll b in higher plants was determined by greening etiolated maize leaves, excised from dark-grown
Abraham R. J. +27 more
core +1 more source
Leaf proteins in five varieties of red clover cultivated in Poland
Protein fractions: albumins, globulins, gluteins and prolamins were extracted from the leaves of 5 varieties of red clover. 'Skrzeszowicka' and 'Hruszowska' showed the highest content of total protein, 'Rotra' however – the highest globulin level ...
W. Maciejewska-Potapczyk +5 more
doaj +1 more source

