A Review of Extraction, Structural Properties, Biological Activities of <i>Dendrobium chrysotoxum</i> Polysaccharides. [PDF]
Overview of extraction, biological activities, and application prospects of DCP. (a) Collection, weighing, shearing, and grinding of D. chrysotoxum stems. (b) Aqueous extraction, ultracentrifugation, drying, and filtration to obtain purified DCP. (c) Mechanistic studies and preclinical or retrospective clinical investigations of DCP bioactivities.
Zhang X, Xu Z, Chen J, Zhang W, Xie L.
europepmc +2 more sources
Milk-Clotting Protease From <i>Bacillus stercoris</i>NCCP-3139: A Potential Microbial Rennet for Cheese Production. [PDF]
A milk‐clotting protease from Bacillus stercoris NCCP‐3139 was isolated from soil and purified via ammonium sulfate precipitation and sequential chromatographic techniques, yielding a homogeneous enzyme confirmed by SDS–PAGE. The protease exhibited high catalytic selectivity, with a marked increase in milk‐clotting‐to‐proteolytic activity ratio (50–550)
Sibtain M +8 more
europepmc +2 more sources
Extraction, Structural Characterization and Anti-Inflammatory Activity of Polysaccharides From <i>Conioselinum vaginatum</i>. [PDF]
This study employed an ultrasonic‐assisted extraction (UAE) method combined with column chromatography purification to obtain two homogeneous polysaccharides (CVP‐I and CVP‐II) from Conioselinum vaginatum, which exhibited distinct physicochemical properties and structural characteristics, and exerted anti‐inflammatory effects by inhibiting the ...
Ren C +6 more
europepmc +2 more sources
Fractionation of Nucleic Acid on Benzoylated-Naphthoylated DEAE Cellulose
Nucleic acids have been fractionated by column chromatography using methylated albumin kieselguhr (Mandell & Hershey, 1960; Sueoka & Cheng, 1962) cellulose CF-11 (Franklin, 1966), agarose (Erikson & Gordon, 1966) and hydroxyapatite (Muench & Berg, 1966).
Sinsheimer, Robert L. +2 more
core +4 more sources
L-ARGININE AND L-GLUTAMIC ACID INCREASE THE CONTENT OF PROTEIN C IN THE EARLY STAGES OF ISOLATION FROM DONOR PLASMA [PDF]
Current large-scale production of blood-derived pharmacological preparations is aimed at expanding the list of products and deeper extraction of target proteins especially at the pre-purification stage.
I. I. Patalakh +2 more
doaj +1 more source
Separation, Purification, Structural Characterization, and In Vitro Hypoglycemic Activity of Polysaccharides from Panax notoginseng Leaves [PDF]
This study optimized the extraction process of crude polysaccharides from Panax notoginseng leaves (PNLP) using the ultrasonic-assisted dual-enzyme method through a single-factor combined with response surface experiment.
Xueling Zhang +7 more
doaj +2 more sources
Comparative Study of the Components and Anti-Fatigue Effect of Polysaccharides from China and Russia
Schisandra chinensis Bail. polysaccharides from China (CSP) and Russia (RSP) were separated by DEAE-52 cellulose column chromatography. The content of neutral polysaccharide was determined by the phenol concentrated sulfuric acid method, the content of ...
Li Han +8 more
doaj +1 more source
Comparative hydrolysis effect of industrial xylanase purified from Bacillus stearothermophilus (ATCC 12980) on two kinds of xylan [PDF]
Xylan the major portion of the hemicellulose of plant cell wall is a heterogeneous polysaccharide. Xylanases(EC:3.2.1.8) are enzymes obtained from different species of microorganisms that degrade the xylosidic linkages of xylan’s backbone producing ...
Zohreh Faezi Zadeh, Amir Gharib
doaj +1 more source
Acid phosphatase and ribonuclease extracted with 0.1 M sodium acetate buffer, pH 5.1 from Dactylis glomerata seeds, and partially purified by means of 70% ethanol precipitation showed electrophoretic and Chromatographic heterogeneity.
Eleonora Wieczorek +2 more
doaj +1 more source
Various cellulose and dextran anion exchangers bind bile salts in vitro under conditions of pH and ionic strength resembling those in the lumen of the small intestine.
Thomas M. Parkinson
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