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Chromatography of Acidic Polysaccharides on Deae-Cellulose [PDF]
Australian Journal of Biological Sciences, 1962 The chromatography of polysaccharides on resinous ion-exchangers has been reported by Steiner, Neukom, and Deuel (1958). Neukom et al. (1960) later reported the chromatography of a variety of wheat and sugar-beet polysaccharides on DEAEcellulose. Only "neutral" polysaccharides could be eluted at pH 6-8 by increasing buffer and salt concentrations; acid
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Shipin gongye ke-jiIn the present study, the fruiting bodies of Cordyceps militaris were used as raw materials to extract the crude polysaccharides Y-1, Y-2, Y-3, Y-4, Y-5, Y-6 and Y-7, which fractional precipitation using the ethanol with different volume fractions of 30%,
Yanan WANG +11 more
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Purification of Rennet by DEAE-Cellulose Chromatography
Journal of the agricultural chemical society of Japan, 1966 レンネットをDEAE-セルロースカラムに吸着させ, 0.1M燐酸緩衝液(pH 5.7)に0.1, 0.2および0.3M NaClを加えて調製した溶出液および0.1N HClで順次溶出させてレンニンを分離精製し,あわせてレンネット中に混在するペプシンをも分離し,その活性割合を測定した. (1) 0.1M燐酸緩衝液(pH 5.7)に添加するNaClの濃度を0.1Mから0.2Mに増加した溶出液で得られるFraction 3に凝乳力カが存在し, pH 6.62,イオン強度0.2の燐酸緩衝液を用いた電気泳動,沈降分析からみて均一性の高いレンニンが得られた.
Umeo YOSHINO +3 more
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Journal of Lipid Research, 1980 Phospholipase B (lysolecithin acyl-hydrolase, EC 3.1.1.5) from the mycelia of Penicillium notatum (Institute for Fermentation, Osaka, Japan; No.4640) was adsorbed from a crude solution to palmitoyl cellulose. Adsorption was efficient at pH 4 at low ionic
S Imamura, Y Horiuti
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The study of correlations between B-Lactamase and protein A in Staphylococcus aureus
Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, 2000 Objective: Staphylococcus aureus is the type species and pathogen of human. There is some factors that determines the virulence of bacteria, but protein A and B-Lactamase are the most important factors.
R Kasra Kermanshahi +2 more
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Biochemistry Research International, 2011 This paper deals with the purification of four proteins from Euphorbia characias latex, a copper amine oxidase, a nucleotide pyrophosphatase/phosphodiesterase, a peroxidase, and a purple acid phosphatase.
Rosaria Medda +3 more
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Phosphatase activity of Poa pratensis seeds. I. Preliminary studies on acid phosphatase II
Acta Societatis Botanicorum Poloniae, 2015 Acid phosphatase (EC 3.1.3.2) was extracted with 0.1 M sodium acetate buffer pH 5.1 from Poa pratensis seeds, and separated into three fractions by chromatography on DEAE cellulose.
I. Lorenc-Kubis, B. Morawiecka
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مجلة مركز بحوث التقنيات الاحيائيةBackground: In the current study the enzyme cytosine deaminase was extracted from the ready-made bread Saccharomyces cerevisiae yeast represented different types from the local market.
Muna A. Mutter +2 more
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Extraction and purification of L-Asparaginase II from local isolate of Proteus vulgaris
مجلة بغداد للعلوم, 2011 Forty one isolates of genus Proteus were collected from 140 clinical specimens such as urine, stool, wound, burn, and ear swabs from patients of both sex. These isolates were identified to three Proteus spp. P. mirabilis, P. vulgaris and P.
Baghdad Science Journal
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Extraction, Purification and Antioxidant Activity of Polysaccharide from White Lilacs
Shipin gongye ke-jiEnzyme-assisted ultrasonic extraction was employed to extract polysaccharides from white lilacs. The effects of various experimental conditions on the yield of polysaccharides from white lilacs were systematically analyzed using the response surface ...
Tianyi WANG +4 more
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