Results 61 to 70 of about 32,605 (213)
Purification of peptidyl-tRNA on benzoylated DEAE-cellulose columns
Nucleic Acids Research, 1975 The o-nitrophenylsulfenyl group, and amino protecting group in the chemical synthesis of peptidyl-tRNA was used to attach newly synthesized peptidyl-tRNA to a benzoylated DEAE-cellulose column. This facilitated the isolation of a highly purified specific tRNA with a well defined peptide chain.
D, Eilat, E, Ziv, Y, Lapidot
openaire +3 more sources
Legume Science, Volume 7, Issue 4, December 2025.ABSTRACT This study reports the purification and characterization of protease inhibitors (PIs) from three Egyptian legumes: soybean (Glycine max), cowpea (Vigna unguiculata), and chickpea (Cicer arietinum). Their antimicrobial and cytotoxic activities were also assessed.
Mona M. Ibrahim +5 more
wiley +1 more source
Characterization of the mitochondrial processing peptidase of Neurospora crassa [PDF]
, 1994 The mitochondrial processing peptidase (MPP) of Neurospora crassa is constituted by an alpha- and a beta-subunit. We have purified alpha-MPP after expression in Escherichia coli while beta-MPP was purified from mitochondria.
Arretz, Michael +4 more
core
Cell Biology International, Volume 49, Issue 11, Page 1395-1410, November 2025.ABSTRACT Chagas disease, caused by the protozoan Trypanosoma cruzi, is a neglected tropical disease with limited treatment options and no available vaccine. Understanding the role of proteins in the parasite's biological cycle is critical for advancing vaccine development and optimizing therapies.
Normanda Souza‐Melo +2 more
wiley +1 more source
Separation of Bovine Serum Albumin Using Chromatographical Column: Parameters and Simulation
Iraqi Journal of Chemical and Petroleum Engineering, 2010 A liquid-solid chromatography of Bovine Serum Albumin (BSA) on (diethylaminoethyl-cellulose) DEAE-cellulose adsorbent is worked experimentally, to study the effect of changing the influent concentration of (0.125, 0.25, 0.5, and 1 mg/ml) at constant ...
Cecilia Khoshaba Haweel +2 more
doaj
Journal of Genetic Engineering and Biotechnology, 2015 Alkaline serine protease was purified to homogeneity from culture supernatant of a thermophilic, alkaliphilic Bacillus sp. by 80% ammonium sulphate precipitation followed by CM-cellulose and DEAE-cellulose ion exchange column chromatography.
Aysha Kamran +4 more
doaj +1 more source
Isolation and characterization of a laminin-binding protein from rat and chick muscle. [PDF]
, 1988 A major laminin-binding protein (LBP), distinct from previously described LBPs, has been isolated from chick and rat skeletal muscle (Mr 56,000 and 66,000, respectively).
Frazer, KA +3 more
core
Electronic Journal of Biotechnology, 2015 Background: The major challenges associated with the fermentation of lignocellulosic hydrolysates are the reduction in the operating cost and minimizing the complexity of the process.
Tatsaporn Todhanakasem +3 more
doaj +1 more source
Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain [PDF]
, 1983 A calcium and calmodulin-dependent protein kinase has been purified from rat brain. It was monitored during the purification by its ability to phosphorylate the synaptic vesicle-associated protein, synapsin I.
Bennett, Mark K. +2 more
core
Engineering in Life Sciences, Volume 25, Issue 10, October 2025.ABSTRACT The growing demand for large quantities of high‐purity lentiviral vectors (LVs) has driven the development of scalable, cost‐effective purification strategies. Membrane chromatography is particularly well‐suited for purifying large biological entities like LVs due to its low mass transfer resistance and minimal back pressure.
Sara Cardoso +3 more
wiley +1 more source