Deep mutational scanning of proteins in mammalian cells [PDF]
Cell Reports: Methods, 2023 Summary: Protein mutagenesis is essential for unveiling the molecular mechanisms underlying protein function in health, disease, and evolution. In the past decade, deep mutational scanning methods have evolved to support the functional analysis of nearly
Stefanie Maes +3 more
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Variant scoring tools for deep mutational scanning [PDF]
Molecular Systems BiologyDeep mutational scanning (DMS) can systematically assess the effects of thousands of genetic variants in a single assay, providing insights into protein function, evolution, host-pathogen interactions, and clinical impacts.
Hasan Çubuk +4 more
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Deep-learning structure elucidation from single-mutant deep mutational scanning [PDF]
Nature CommunicationsDeep learning has revolutionized the field of protein structure prediction. AlphaFold2, a deep neural network, vastly outperformed previous algorithms to provide near atomic-level accuracy when predicting protein structures.
Zachary C. Drake +3 more
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Deep Mutational Scanning in Immunology: Techniques and Applications [PDF]
PathogensMutations may cause changes in the structure and function of immune-related proteins, thereby affecting the operation of the immune system. Deep mutational scanning combines saturation mutagenesis, functional selection, and high-throughput sequencing to ...
Chengwei Shao +3 more
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Applications of Deep Mutational Scanning in Virology [PDF]
Viruses, 2021 Several recently developed high-throughput techniques have changed the field of molecular virology. For example, proteomics studies reveal complete interactomes of a viral protein, genome-wide CRISPR knockout and activation screens probe the importance ...
Thomas D. Burton, Nicholas S. Eyre
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Deep mutational scanning of H5 hemagglutinin to inform influenza virus surveillance. [PDF]
PLoS BiologyH5 influenza is considered a potential pandemic threat. Recently, H5 viruses belonging to clade 2.3.4.4b have caused large outbreaks in avian and multiple nonhuman mammalian species.
Bernadeta Dadonaite +14 more
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Deep mutational scanning and CRISPR-engineered viruses: tools for evolutionary and functional genomics studies [PDF]
mSphereRecent advancements in synthetic biology and sequencing technologies have revolutionized the ability to manipulate viral genomes with unparalleled precision.
Mercedes Paz, Gonzalo Moratorio
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Deep mutational scanning quantifies DNA binding and predicts clinical outcomes of PAX6 variants [PDF]
Molecular Systems BiologyNonsense and missense mutations in the transcription factor PAX6 cause a wide range of eye development defects, including aniridia, microphthalmia and coloboma.
Alexander F McDonnell +8 more
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Deep mutational scanning: A versatile tool in systematically mapping genotypes to phenotypes
Frontiers in Genetics, 2023 Unveiling how genetic variations lead to phenotypic variations is one of the key questions in evolutionary biology, genetics, and biomedical research.
Huijin Wei +4 more
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Synergistic effects of targeted PI3K signaling inhibition and chemotherapy in liposarcoma. [PDF]
, 2014 While liposarcoma is the second most common soft tissue malignant tumor, the molecular pathogenesis in this malignancy is poorly understood. Our goal was therefore to expand the understanding of molecular mechanisms that drive liposarcoma and identify ...
Borger, Darrell R +12 more
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