Results 91 to 100 of about 19,763 (281)
Identifying Residue‐Residue Contacts via Deep Mutational Scanning
The FASEB Journal, 2018 Structural knowledge is critical for understanding a protein's biological function and interactions, aiding drug design and other advances in medicine and biotechnology. However, there are many proteins for which structural data are either of poor quality or completely absent, and the inefficiency of experimental structure ...
Emily C. Hinds, Philip A. Romero
openaire +1 more source
Annotating Protein Functional Residues by Coupling High-Throughput Fitness Profile and Homologous-Structure Analysis. [PDF]
, 2016 Identification and annotation of functional residues are fundamental questions in protein sequence analysis. Sequence and structure conservation provides valuable information to tackle these questions.
Du, Yushen +7 more
core +3 more sources
Molecular Oncology, EarlyView.Clinical trials on PARP inhibitors in urothelial carcinoma (UC) showed limited efficacy and a lack of predictive biomarkers. We propose SLFN5, SLFN11, and OAS1 as UC‐specific response predictors. We suggest Talazoparib as the better PARP inhibitor for UC than Olaparib.
Jutta Schmitz +15 more
wiley +1 more source
Journal of Open Source Software, 2023 Summary and PurposeUnderstanding how mutations impact a protein’s functions is valuable for many types of biological questions. High-throughput techniques such as deep-mutational scanning (DMS) have greatly expanded the number of mutation-function datasets.
William W. Hannon, Jesse D. Bloom
openaire +2 more sources
A comprehensive map of human glucokinase variant activity
Genome Biology, 2023 Background Glucokinase (GCK) regulates insulin secretion to maintain appropriate blood glucose levels. Sequence variants can alter GCK activity to cause hyperinsulinemic hypoglycemia or hyperglycemia associated with GCK-maturity-onset diabetes of the ...
Sarah Gersing +14 more
doaj +1 more source
Using cellular fitness to map the structure and function of a major facilitator superfamily effluxer. [PDF]
, 2017 The major facilitator superfamily (MFS) effluxers are prominent mediators of antimicrobial resistance. The biochemical characterization of MFS proteins is hindered by their complex membrane environment that makes in vitro biochemical analysis challenging.
Bennett, Matthew R +5 more
core +2 more sources
Molecular Oncology, EarlyView.HDAC4 is degraded by the E3 ligase FBXW7. In colorectal cancer, FBXW7 mutations prevent HDAC4 degradation, leading to oxaliplatin resistance. Forced degradation of HDAC4 using a PROTAC compound restores drug sensitivity by resetting the super‐enhancer landscape, reprogramming the epigenetic state of FBXW7‐mutated cells to resemble oxaliplatin ...
Vanessa Tolotto +13 more
wiley +1 more source
Deep mutational scanning reveals the structural basis for α-synuclein activity
Nature Chemical Biology, 2020 Defining the biologically active structures of proteins in their cellular environments remains challenging for proteins with multiple conformations and functions, where only a minor conformer might be associated with a given function. Here, we use deep mutational scanning to probe the structure and dynamics of α-synuclein, a protein known to adopt ...
Robert W. Newberry +4 more
openaire +5 more sources
A framework for exhaustively mapping functional missense variants
Molecular Systems Biology, 2017 Although we now routinely sequence human genomes, we can confidently identify only a fraction of the sequence variants that have a functional impact. Here, we developed a deep mutational scanning framework that produces exhaustive maps for human missense
Jochen Weile +20 more
doaj +1 more source
The potential for liquid biopsies in the precision medical treatment of breast cancer. [PDF]
, 2016 Currently the clinical management of breast cancer relies on relatively few prognostic/predictive clinical markers (estrogen receptor, progesterone receptor, HER2), based on primary tumor biology.
Barrak, Dany K +5 more
core +1 more source